Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yefM-yoeB (relBE)/Txe-RelB |
| Location | 1780..2281 | Replicon | plasmid pRHBSTW-00570_4 |
| Accession | NZ_CP056402 | ||
| Organism | Citrobacter sp. RHBSTW-00570 | ||
Toxin (Protein)
| Gene name | yoeB | Uniprot ID | A0A7L6INF2 |
| Locus tag | HV237_RS25810 | Protein ID | WP_072058806.1 |
| Coordinates | 2006..2281 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | yefM | Uniprot ID | A0A7L6INP2 |
| Locus tag | HV237_RS25805 | Protein ID | WP_047359280.1 |
| Coordinates | 1780..2025 (+) | Length | 82 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HV237_RS25800 | 1144..1350 | + | 207 | WP_047359279.1 | hypothetical protein | - |
| HV237_RS25805 | 1780..2025 | + | 246 | WP_047359280.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| HV237_RS25810 | 2006..2281 | + | 276 | WP_072058806.1 | Txe/YoeB family addiction module toxin | Toxin |
| HV237_RS25815 | 2452..3432 | - | 981 | WP_000019452.1 | IS5-like element ISKpn26 family transposase | - |
| HV237_RS25820 | 3583..4476 | + | 894 | WP_047359282.1 | GalU regulator GalF | - |
| HV237_RS25825 | 4822..5802 | - | 981 | WP_181541876.1 | IS5 family transposase | - |
| HV237_RS25830 | 5895..6311 | + | 417 | WP_052951298.1 | hypothetical protein | - |
| HV237_RS25835 | 6311..7117 | + | 807 | WP_047359263.1 | glycosyltransferase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | - | galF | 1..34198 | 34198 | |
| - | inside | IScluster/Tn | - | galF | 49..5802 | 5753 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10856.34 Da Isoelectric Point: 10.3145
>T163421 WP_072058806.1 NZ_CP056402:2006-2281 [Citrobacter sp. RHBSTW-00570]
MSKWNIKFTKNGADDINYWRGTDKKKFERIRQLLHSIESDPLTGIGKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEII
IYAARYHYGDK
MSKWNIKFTKNGADDINYWRGTDKKKFERIRQLLHSIESDPLTGIGKPERLRHHKDPALYSRRIDQSHRLVYSIQNGEII
IYAARYHYGDK
Download Length: 276 bp
>T163421 NZ_CP072565:2677345-2677452 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA
Antitoxin
Download Length: 82 a.a. Molecular weight: 9276.43 Da Isoelectric Point: 4.5069
>AT163421 WP_047359280.1 NZ_CP056402:1780-2025 [Citrobacter sp. RHBSTW-00570]
MHAITFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAAHINESMKQAERGEFVEVE
Y
MHAITFTDARKHFADTMKRVTDDAEPVRVMRRDAPDIVMIDAGEYEALIETVYLFSNPANAAHINESMKQAERGEFVEVE
Y
Download Length: 246 bp
>AT163421 NZ_CP072565:c2677298-2677232 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7L6INF2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7L6INP2 |