Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4917938..4918698 Replicon chromosome
Accession NZ_CP056399
Organism Citrobacter sp. RHBSTW-00570

Toxin (Protein)


Gene name higB Uniprot ID A0A7W3E2Q1
Locus tag HV237_RS23310 Protein ID WP_047357780.1
Coordinates 4917938..4918249 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7W3E2R1
Locus tag HV237_RS23315 Protein ID WP_047357781.1
Coordinates 4918246..4918698 (+) Length 151 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV237_RS23280 4913605..4914507 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV237_RS23285 4914504..4915139 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV237_RS23290 4915136..4916065 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV237_RS23295 4916102..4916476 - 375 WP_016155182.1 type II toxin-antitoxin system VapC family toxin -
HV237_RS23300 4916476..4916718 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein -
HV237_RS23305 4916924..4917853 + 930 WP_047357779.1 alpha/beta hydrolase -
HV237_RS23310 4917938..4918249 + 312 WP_047357780.1 type II toxin-antitoxin system HigB family toxin Toxin
HV237_RS23315 4918246..4918698 + 453 WP_047357781.1 helix-turn-helix domain-containing protein Antitoxin
HV237_RS23320 4918716..4919657 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV237_RS23325 4919702..4920139 - 438 WP_016157874.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV237_RS23330 4920136..4921008 - 873 WP_019077938.1 virulence factor BrkB family protein -
HV237_RS23335 4921002..4921601 - 600 WP_016157873.1 glucose-1-phosphatase -
HV237_RS23340 4921869..4922897 - 1029 WP_047357782.1 LacI family DNA-binding transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4916924..4941455 24531


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(95-148)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12545.70 Da        Isoelectric Point: 10.1490

>T163418 WP_047357780.1 NZ_CP056399:4917938-4918249 [Citrobacter sp. RHBSTW-00570]
MHVISRKPFNEARLRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Download         Length: 312 bp

>T163418 NZ_CP072565:2386980-2388302 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCAAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGCTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACAGCACTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCA
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAAGCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin


Download         Length: 151 a.a.        Molecular weight: 16986.38 Da        Isoelectric Point: 6.1421

>AT163418 WP_047357781.1 NZ_CP056399:4918246-4918698 [Citrobacter sp. RHBSTW-00570]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQHGLKAADLANELGSKSNVSNILNGRRALTISHIKMLAERFNLPADAFIE

Download         Length: 453 bp

>AT163418 NZ_CP072565:2386714-2386980 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGTATTAAGCAGGCGACGATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAAATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E2Q1

Antitoxin

Source ID Structure
AlphaFold DB A0A7W3E2R1

References