Detailed information of TA system TA163384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 400702..401368
• Replicon: chromosome
• Accession: NZ_CP056395
• Organism: Citrobacter freundii strain RHBSTW-00589

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV241_RS01865
• Protein ID: WP_117342464.1
• Coordinates: 401051..401368 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HV241_RS01860
• Protein ID: WP_003837894.1
• Coordinates: 400702..400998 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV241_RS01845	397878..398351	-	474	WP_005121627.1	transcription elongation factor GreB	-
HV241_RS01850	398578..399297	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV241_RS01855	399294..400646	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HV241_RS01860	400702..400998	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HV241_RS01865	401051..401368	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV241_RS01870	401492..403114	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV241_RS01875	403493..405211	+	1719	WP_181624909.1	DUF4153 domain-containing protein	-
HV241_RS01880	405262..406140	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T163384 WP_117342464.1 NZ_CP056395:c401368-401051 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T163384 NZ_CP072559:2800460-2800837 [Pseudomonas sp. BYT-1]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT163384 WP_003837894.1 NZ_CP056395:c400998-400702 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT163384 NZ_CP072559:2800818-2801153 [Pseudomonas sp. BYT-1]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5