Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 358127..358773 Replicon chromosome
Accession NZ_CP056372
Organism Klebsiella pneumoniae strain RHBSTW-00636

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag HV253_RS01655 Protein ID WP_016529833.1
Coordinates 358127..358474 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag HV253_RS01660 Protein ID WP_002920557.1
Coordinates 358474..358773 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV253_RS01645 354053..355486 + 1434 WP_002920564.1 glycogen synthase GlgA -
HV253_RS01650 355504..357951 + 2448 WP_002920561.1 glycogen phosphorylase -
HV253_RS01655 358127..358474 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV253_RS01660 358474..358773 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
HV253_RS01665 358836..360344 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
HV253_RS01670 360549..360878 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
HV253_RS01675 360929..361759 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
HV253_RS01680 361809..362567 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T163328 WP_016529833.1 NZ_CP056372:358127-358474 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T163328 NZ_CP072539:c145577-145236 [Escherichia coli O128ac:H12]
TTGAAAATACTACCTGCTACAACTTCGCGGGCGGCGAAGCCCTGCCTGTCACCCGTTGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGTCTTACCCTAAACGATACTCCTTTCAGTGATGAAACCGTTATTCAGAATCACATTA
ATGCCGGGATCACGCTGGCCGATGCTGTTAACTTTCTGGTGGAAAAATACGAACTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCCCCTTATCTTCGGGCTGTAGATATCCTGCGGGCAAGACAGGCAACTGGCCTTTTACGACAAAG
CCGTAACACCGCAGTACGGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT163328 WP_002920557.1 NZ_CP056372:358474-358773 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT163328 NZ_CP072539:c145915-145598 [Escherichia coli O128ac:H12]
ATGAGCAACATCACTTGGGGCCTGCAGCGCAATATCACGCCGTGCCTGGGAGCCCGCCTTGTGCAGGAGGGGAACCGGCT
GCACTACCTGGCTGACCGGACCAGTATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAGTCGATGCTTACCACTGGTGAAATGAACCCTCACCATGCCCACTACGTAATCCTGTATCAC
AACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTATGTATACGTCGCTGTTTATTCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References