Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 99501..100228 Replicon plasmid pRHBSTW-00658_2
Accession NZ_CP056366
Organism Citrobacter freundii strain RHBSTW-00658

Toxin (Protein)


Gene name higB Uniprot ID A0A663AUB1
Locus tag HV257_RS24560 Protein ID WP_000558568.1
Coordinates 99501..99812 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7U3M0L1
Locus tag HV257_RS24565 Protein ID WP_072199090.1
Coordinates 99809..100228 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV257_RS24535 95298..97043 + 1746 WP_004187005.1 ATP-grasp domain-containing protein -
HV257_RS24540 97356..97427 - 72 Protein_100 cellulose synthase -
HV257_RS24545 97474..98442 - 969 WP_074132802.1 IS5 family transposase -
HV257_RS24550 98465..98860 + 396 Protein_102 lactate utilization protein C -
HV257_RS24555 98910..99290 - 381 Protein_103 gluconate permease -
HV257_RS24560 99501..99812 + 312 WP_000558568.1 type II toxin-antitoxin system HigB family toxin Toxin
HV257_RS24565 99809..100228 + 420 WP_072199090.1 helix-turn-helix domain-containing protein Antitoxin
HV257_RS24570 100265..100474 - 210 WP_020806042.1 hypothetical protein -
HV257_RS24575 101049..102053 - 1005 WP_000427623.1 IS110-like element IS4321 family transposase -
HV257_RS24580 102228..102653 - 426 WP_023316432.1 glutaredoxin-dependent arsenate reductase -
HV257_RS24585 102666..103955 - 1290 WP_032409266.1 arsenic transporter -
HV257_RS24590 104000..104296 - 297 WP_047721846.1 metalloregulator ArsR/SmtB family transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - - 1..109317 109317
- inside IScluster/Tn - - 97474..105400 7926


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12372.11 Da        Isoelectric Point: 10.0935

>T163326 WP_000558568.1 NZ_CP056366:99501-99812 [Citrobacter freundii]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T163326 NZ_CP072528:c91790-91503 [Acinetobacter baumannii]
ATGGAACAGTATTTCGAATGGGATGAGGCAAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTTGAAACTGCAAG
CCTTGTTTTTGAAGATCCTCTACGGATCTCAATCCAAGACAGACATACCAATGGGGAAGAGCGTTGGCAAACCATTGGTA
AGGTTAAAGGCGTACTGATGTTATTAGTCGCTCATACCATCTTTGATGAAGATGACTGTGAAATCATACGAATCATTAGT
GCAAGACAAGTAACCAAAGCGGAGCGCAATCAATATGAGCATGGTTAG

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15592.75 Da        Isoelectric Point: 4.4075

>AT163326 WP_072199090.1 NZ_CP056366:99809-100228 [Citrobacter freundii]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIEALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT163326 NZ_CP072528:c91516-91205 [Acinetobacter baumannii]
ATGAGCATGGTTAGATATACACGCGAAGAGCTGAATGAAAATTTCAGCAACAAGCAAGATGCTGAAGTTGAACACTTGCT
TGCTAAGGGAACGGTTCCTGACGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGTAATGCTATTCGTC
ATGGGCAATTTTTTCGTCCAGTGAAGCAACAAACGTCAGTTCGTCTTGATGCGGATGTATTAGCATGGTTAAAAGCACAA
GGAAAAGGTTATCAAACACGTATGAATAAAATTCTGCGTGAAGCAATGCTAAAAGATTTAAAAAATCATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AUB1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U3M0L1

References