Detailed information of TA system TA163287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4555435..4556105
• Replicon: chromosome
• Accession: NZ_CP056314
• Organism: Citrobacter freundii strain RHBSTW-00830

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6N6JXY6
• Locus tag: HV290_RS21715
• Protein ID: WP_046671374.1
• Coordinates: 4555803..4556105 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV290_RS21710
• Protein ID: WP_046671375.1
• Coordinates: 4555435..4555776 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV290_RS21680	4550976..4551734	+	759	WP_003844769.1	phosphonate C-P lyase system protein PhnK	-
HV290_RS21685	4551824..4552504	+	681	WP_086529982.1	phosphonate C-P lyase system protein PhnL	-
HV290_RS21690	4552501..4553637	+	1137	WP_003031811.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV290_RS21695	4553640..4554194	+	555	WP_003841102.1	ribose 1,5-bisphosphokinase	-
HV290_RS21700	4554181..4554615	+	435	WP_003031809.1	aminoalkylphosphonate N-acetyltransferase	-
HV290_RS21705	4554624..4555382	+	759	WP_086529671.1	phosphonate metabolism protein PhnP	-
HV290_RS21710	4555435..4555776	-	342	WP_046671375.1	HigA family addiction module antidote protein	Antitoxin
HV290_RS21715	4555803..4556105	-	303	WP_046671374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV290_RS21720	4556261..4556590	-	330	WP_181523195.1	hypothetical protein	-
HV290_RS21725	4556660..4558924	-	2265	WP_086529672.1	hybrid sensor histidine kinase/response regulator	-
HV290_RS21730	4559039..4560562	+	1524	WP_086529673.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11819.43 Da
• Isoelectric Point: 9.9581

>T163287 WP_046671374.1 NZ_CP056314:c4556105-4555803 [Citrobacter freundii]
MSKSLNIRSFRDTWLEDFFERATSHRKIPADIHTALARKLDIINAAVSHRDLRSPPGNRYEELTGKLQEYSSIRVNKQYR
LIFKWVNGKAEDVYLDPHIY

Nucleotide

• Length: 303 bp

>T163287 NZ_CP072496:895306-895716 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13017.84 Da
• Isoelectric Point: 7.2040

>AT163287 WP_046671375.1 NZ_CP056314:c4555776-4555435 [Citrobacter freundii]
MKQATRKPTTVGDILQYEYLEPLGLKINDLAEILHVHRNTVSALVNNNRKLTTDMAFRLAKAFDTSVDFWRNLQAAVDLW
EVENDMRAQEELNRIVSVKDFIAQRNSESKKVA

Nucleotide

• Length: 342 bp

>AT163287 NZ_CP072496:895059-895325 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A6N6JXY6