Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 49659..50386 Replicon plasmid pRHBSTW-00843_2
Accession NZ_CP056302
Organism Klebsiella pneumoniae strain RHBSTW-00843

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag HV294_RS25735 Protein ID WP_004118600.1
Coordinates 49659..49970 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag HV294_RS25740 Protein ID WP_000990392.1
Coordinates 49967..50386 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV294_RS25715 46173..46892 + 720 WP_001102107.1 (Fe-S)-binding protein -
HV294_RS25720 46903..48330 + 1428 WP_000023895.1 iron-sulfur cluster-binding protein -
HV294_RS25725 48323..49018 + 696 WP_004118595.1 lactate utilization protein C -
HV294_RS25730 49068..49448 - 381 Protein_44 gluconate permease -
HV294_RS25735 49659..49970 + 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
HV294_RS25740 49967..50386 + 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
HV294_RS25745 50423..51625 - 1203 WP_003031541.1 hypothetical protein -
HV294_RS25750 51618..51947 - 330 WP_094309446.1 baseplate protein -
HV294_RS25755 51944..52603 - 660 WP_000078540.1 hypothetical protein -
HV294_RS25760 53027..53620 - 594 WP_044348674.1 hypothetical protein -
HV294_RS25765 53936..54289 + 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
HV294_RS25770 54337..54699 + 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / blaSHV-12 / dfrA1 / ant(3'')-Ia / qacE / sul1 - 1..267374 267374


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T163246 WP_004118600.1 NZ_CP056302:49659-49970 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T163246 NZ_CP072467:c452344-452102 [Spiractinospora alimapuensis]
GTGGCGCGGGCACTCTCAAACCGGCTTCCGCAAAAGGTCGCCCACGCGGTCTGCGAGTTCATCACATCGGTACTACTGGA
TAATCCCCGTCGTGTAGGAAAGGAACTCCTGTTGCCGCCCTACGACGGAACATATTCCGCGCGTCGCGGCACATACCGCG
TTCTGTATGGGATCGATGACGAGTCTCGGACGGTCACAGTTACCGCAGTCGAACACCGGGGAGACGCCTACCGGTCCCGC
TGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT163246 WP_000990392.1 NZ_CP056302:49967-50386 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT163246 NZ_CP072467:c452660-452382 [Spiractinospora alimapuensis]
ATGGAGACATTGCCCCTCACCGAGGCACGCAATCGATTGTCCGAACTCGTCGATGACGTACAGCGCACGCATCAACGCGT
CACCATCACCCGACACGGTGAGGTCGCCGCGGTCATCATGTCCGCCGACGATCTGGACGCCTTGGAGGAGACGTTGGAGG
TACTCAGCAGTCCGGAGCTCATGGGCCAACTCGCCGAGTCCAAAATCGCCCGAGAGCAGGGTGATGTTCTCGACGTCGAT
CAGCTTCGATCCCTGATGGCCGAGCATCGCGGTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References