Detailed information of TA system TA163190

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 328790..329376
• Replicon: chromosome
• Accession: NZ_CP056275
• Organism: Klebsiella pneumoniae strain RHBSTW-00867

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HV307_RS01535
• Protein ID: WP_023285605.1
• Coordinates: 329008..329376 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: HV307_RS01530
• Protein ID: WP_004174006.1
• Coordinates: 328790..329011 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV307_RS01510	324947..325873	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV307_RS01515	325870..327147	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
HV307_RS01520	327144..327911	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV307_RS01525	327913..328626	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV307_RS01530	328790..329011	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV307_RS01535	329008..329376	+	369	WP_023285605.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV307_RS01540	329649..330965	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV307_RS01545	331072..331959	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV307_RS01550	331956..332801	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV307_RS01555	332803..333873	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_009648 Klebsiella pneumoniae subsp. pneumoniae MGH 78578 Prophage TA022291 4164800..4173540 0.009	inside	Prophage	-	-	327144..334610	7466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13534.89 Da
• Isoelectric Point: 8.6410

>T163190 WP_023285605.1 NZ_CP056275:329008-329376 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGITVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T163190 NZ_CP072455:591597-592541 [Xenorhabdus budapestensis]
ATGAAAAAATTAAAAGATCAATTCTTAGAGGTTTCCGATGCCTTAGAGCTCGGAAACCCAGCCATTATTGAAAAAGATTA
CTGGGTAGTGGCTTTGCTTGCTGAGTTAGCAAAGCTTGCTTTTGATACGCATCAAATGGTTTTTTCTGGCGGGACGGCAC
TGGCTAAATCCAATGTAAAAATCCTCAGGATGTCGGAAGATGTCGACATCAAACTCATCCCAACGGATGCATTTAACTCA
TTCGGTCGTGACAAGCGCAAGGCGACACGTAAAGCTCTGGTGCAACAAATCGAAGAATCTCTAATTCACCAATCAAGCCA
TTTTTCTATTAAAGAAAAAAAAGCCCGTGATGAATACCGCTACGTCGAATATGAACTGAAATACCCACAGCAATTTAGAC
AAGCACCTTGCTTGCGCCCAATCATTAAACTGGAACTCATGGAGAGCATACCTCTGCTTCAAATAGAACAGCGTAGCATT
CAATCACTGGTTGCTGAGCTGTATCATCAGCCTCACGAAGTCAACGCATTTTCTTGTATCTCCGTTCAATCCACTTTGGT
GGAGAAAATCATTTCTATGCTGCGCCGTACCATGCTAGTCAAACGAGATTCTGAGCGCAAAGATGATGCTACGTTGATAC
GCCATATCTACGATGTACATTGTATTGACGTAGACCAGAAACTTGATTTTGAAACTCTTATGCCCCTTTTCCAAACCGTG
TTACAAGAAGACATTAAGCGATTTGGTAATCAGCATGCGGAGTTTGTTGCTAACCCTGTTCAAGAATTACAGCTAGGTTT
GCAAGAGCTAGAGTCTAACCCGCTGTTTTGTAAAAGGTTTACAGACTTTGTCACCCCAATGGTGTTTAATACAGAAACAC
CCGATTTCGATACCTGCTTTGCCTCATTTAAGCGCATTGCTCAGTCATTGATGAATAATAATTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT163190 WP_004174006.1 NZ_CP056275:328790-329011 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT163190 NZ_CP072455:591190-591594 [Xenorhabdus budapestensis]
ATGACAGCAGCAGATCGAATCAATCAAAAGTTGAAACGTTCGAGACGTTACGTCTTTGAACGCAAAGATTTTGAAGACAT
TGCCAGTTATGACCAGATCGGCCGTGCGCTTAATCAATTGATCAAGCAAGGTGAACTGATGAAAATCGGATATGGCCTGT
ATACTAAAGCACGTAAGAACAGCCTCACAGGTCGCCTAATGCCAACGAATCCTGGTGGCACCGATGCGATTTTACGTGAA
ATTCTTAAGATGAAAGGCGTAGACTTCGAAGTAGATAGTCTATCGTTGCAAAGTCTTTCCGGTAATAGCACACAAATCCC
TTCATCTGTGCAGTATTCGTGGAATCCTAAGCAGTTTAATCGCAAGCTGGTTGTTGGTAATCGCGTACTTAACTCACCAA
GTTAA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7