Detailed information of TA system TA163166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 83097..83960
• Replicon: plasmid pRHBSTW-00902_3
• Accession: NZ_CP056258
• Organism: Citrobacter freundii strain RHBSTW-00902

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: HV319_RS26455
• Protein ID: WP_127785371.1
• Coordinates: 83097..83591 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HV319_RS26460
• Protein ID: WP_079940153.1
• Coordinates: 83616..83960 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV319_RS26425	78121..78669	-	549	WP_127785946.1	DUF1380 family protein	-
HV319_RS26430	79155..79934	-	780	WP_153983909.1	hypothetical protein	-
HV319_RS26435	80094..80942	-	849	WP_044702681.1	DUF4942 domain-containing protein	-
HV319_RS26440	81060..81275	-	216	WP_044702680.1	hypothetical protein	-
HV319_RS26445	81344..82024	-	681	WP_153983908.1	hypothetical protein	-
HV319_RS26450	82122..82370	-	249	WP_136138507.1	hypothetical protein	-
HV319_RS26455	83097..83591	-	495	WP_127785371.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HV319_RS26460	83616..83960	-	345	WP_079940153.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HV319_RS26465	84306..84524	-	219	WP_153983907.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV319_RS26470	84777..85007	-	231	WP_044702671.1	hypothetical protein	-
HV319_RS26475	84998..85261	-	264	WP_044702670.1	DUF1380 family protein	-
HV319_RS26480	85368..85649	-	282	WP_044702669.1	hypothetical protein	-
HV319_RS26485	85797..86315	-	519	WP_079940146.1	hypothetical protein	-
HV319_RS26490	86305..86679	-	375	WP_079940147.1	hypothetical protein	-
HV319_RS26495	86689..87018	-	330	WP_079940148.1	hypothetical protein	-
HV319_RS26500	87032..87382	-	351	WP_079940149.1	hypothetical protein	-
HV319_RS26505	87397..87828	-	432	WP_153983905.1	antirestriction protein	-
HV319_RS26510	88012..88443	-	432	WP_079940151.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..100185	100185

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (11-147)

Antitoxin

• PrlF antitoxin (16-114)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 19100.97 Da
• Isoelectric Point: 8.6573

>T163166 WP_127785371.1 NZ_CP056258:c83591-83097 [Citrobacter freundii]
MEYLEINGWKICFHKCFLDQIVELAQKVAELKAAKPSEYHTKKETKLLRAIERVIEEWIASDPLNPQYRQGETLGDEYKH
WFRVKFLQQFRLFFRCSQEHKTIIIGWVNDFGTLRAYGSKTDAYKVFAAMLNAGNPPDDWDKLLNAATKATAKASIPDFL
TRQP

Nucleotide

• Length: 495 bp

>T163166 NZ_CP072422:c2693332-2692010 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCGGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGAGAGCAGATGTTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12535.20 Da
• Isoelectric Point: 4.4227

>AT163166 WP_079940153.1 NZ_CP056258:c83960-83616 [Citrobacter freundii]
MATQILNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGESIHYTLLAGGKVIMSRKDEEQDDPVVSQFLAFLENDMKK
NPQNIAPVPAFFWESIKALTAGVEVDLDAPLTDD

Nucleotide

• Length: 345 bp

>AT163166 NZ_CP072422:c2693598-2693332 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGATGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA