Detailed information of TA system TA163140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3318020..3318680
• Replicon: chromosome
• Accession: NZ_CP056251
• Organism: Citrobacter sp. RHBSTW-00903

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: HV320_RS16675
• Protein ID: WP_053390179.1
• Coordinates: 3318327..3318680 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV320_RS16670
• Protein ID: WP_103283718.1
• Coordinates: 3318020..3318322 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV320_RS16655	3314688..3315950	+	1263	WP_103283720.1	integrase arm-type DNA-binding domain-containing protein	-
HV320_RS16660	3316632..3317519	-	888	WP_103283719.1	integrase domain-containing protein	-
HV320_RS16665	3317651..3317953	+	303	WP_146053242.1	hypothetical protein	-
HV320_RS16670	3318020..3318322	-	303	WP_103283718.1	XRE family transcriptional regulator	Antitoxin
HV320_RS16675	3318327..3318680	-	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV320_RS16680	3319179..3319367	-	189	WP_181533336.1	helix-turn-helix domain-containing protein	-
HV320_RS16685	3319657..3320148	-	492	WP_088544847.1	hypothetical protein	-
HV320_RS16690	3320380..3320616	+	237	WP_023277393.1	helix-turn-helix domain-containing protein	-
HV320_RS16695	3320616..3322598	+	1983	WP_181533337.1	Mu transposase C-terminal domain-containing protein	-
HV320_RS16700	3322694..3323632	+	939	WP_061068291.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T163140 WP_053390179.1 NZ_CP056251:c3318680-3318327 [Citrobacter sp. RHBSTW-00903]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T163140 NZ_CP072403:c4661062-4660529 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGATTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11054.88 Da
• Isoelectric Point: 6.4738

>AT163140 WP_103283718.1 NZ_CP056251:c3318322-3318020 [Citrobacter sp. RHBSTW-00903]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDIELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT163140 NZ_CP072403:c4661339-4661073 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S535