Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2562662..2563224 Replicon chromosome
Accession NZ_CP056245
Organism Citrobacter freundii strain RHBSTW-00915

Toxin (Protein)


Gene name higB Uniprot ID A0A8B5QCT4
Locus tag HV325_RS12605 Protein ID WP_003836234.1
Coordinates 2562946..2563224 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B5QC60
Locus tag HV325_RS12600 Protein ID WP_003836231.1
Coordinates 2562662..2562946 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV325_RS12585 2560353..2561237 + 885 WP_003833024.1 formate dehydrogenase N subunit beta -
HV325_RS12590 2561230..2561886 + 657 WP_003020054.1 formate dehydrogenase-N subunit gamma -
HV325_RS12595 2562016..2562618 + 603 WP_049016493.1 inorganic diphosphatase -
HV325_RS12600 2562662..2562946 - 285 WP_003836231.1 HigA family addiction module antidote protein Antitoxin
HV325_RS12605 2562946..2563224 - 279 WP_003836234.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV325_RS12610 2563445..2565160 - 1716 WP_049016492.1 ATP-binding cassette domain-containing protein -
HV325_RS12615 2565470..2567167 - 1698 WP_003836239.1 malate dehydrogenase -
HV325_RS12620 2567347..2567487 - 141 WP_003020039.1 stationary-phase-induced ribosome-associated protein -
HV325_RS12625 2567715..2568146 + 432 WP_049016491.1 OsmC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-83)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10637.28 Da        Isoelectric Point: 9.8965

>T163121 WP_003836234.1 NZ_CP056245:c2563224-2562946 [Citrobacter freundii]
MIMNFRHKGLRDLFLHGRTSGVMAKHVRRLRHRLAVIDAASKVTDINMPGYKLHPLVGDRDGIWAIAVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T163121 NZ_CP072393:c4451658-4451524 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGTGCAATTGTGCTGCTGGTGCTGTCGATGTTGTCGGTGGGAATGCTGGCGGGTTGCAACACCGTCGCAGGTGC
CGGCAAGGACGTGCAGGGCGCTGGCGAGAAGGTGGAAGACGCAGCGCGTAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10358.10 Da        Isoelectric Point: 8.8094

>AT163121 WP_003836231.1 NZ_CP056245:c2562946-2562662 [Citrobacter freundii]
MKMANHPRPGDIIQEALEELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQTTWSLAEAQ
KSVDISRLRKLAVQ

Download         Length: 285 bp

>AT163121 NZ_CP072393:c4451885-4451733 [Xanthomonas phaseoli pv. phaseoli]
ATGAAGCGACTGCTGACACTGATGGTGCTGGGCCTGTTTTCGTCCGGCGTGATGACGGGCTGCAACACCGTGGCCGGCGC
CGGCAAGGACATGCAGGGTGCGGGCAACAAGGTCGAGAAAACGGCCGAAAAGTGCAGCGACGGTAAGTGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B5QCT4

Antitoxin

Source ID Structure
AlphaFold DB A0A8B5QC60

References