Detailed information of TA system TA163093

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 324814..325480
• Replicon: chromosome
• Accession: NZ_CP056244
• Organism: Citrobacter freundii strain RHBSTW-00923

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV327_RS01520
• Protein ID: WP_117342464.1
• Coordinates: 325163..325480 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A4U6IRD5
• Locus tag: HV327_RS01515
• Protein ID: WP_003837894.1
• Coordinates: 324814..325110 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV327_RS01500	321990..322463	-	474	WP_005121627.1	transcription elongation factor GreB	-
HV327_RS01505	322690..323409	+	720	WP_001157751.1	two-component system response regulator OmpR	-
HV327_RS01510	323406..324758	+	1353	WP_096759268.1	two-component system sensor histidine kinase EnvZ	-
HV327_RS01515	324814..325110	-	297	WP_003837894.1	helix-turn-helix domain-containing protein	Antitoxin
HV327_RS01520	325163..325480	-	318	WP_117342464.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV327_RS01525	325604..327226	-	1623	WP_115257300.1	phosphoenolpyruvate carboxykinase (ATP)	-
HV327_RS01530	327605..329323	+	1719	WP_181517782.1	DUF4153 domain-containing protein	-
HV327_RS01535	329374..330252	-	879	WP_096759272.1	Hsp33 family molecular chaperone HslO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12226.13 Da
• Isoelectric Point: 9.8310

>T163093 WP_117342464.1 NZ_CP056244:c325480-325163 [Citrobacter freundii]
MFTFIELQGFSKRRPLLLPDDEFRAFQEALIENPEAGDTIAGTGGFRKIRWSRSGMGKRSGIRVIYYNVTRKGRIYLALL
YPKNEQDDLTEEQKRVLMHLSDMLI

Nucleotide

• Length: 318 bp

>T163093 NZ_CP072377:c1212019-1211762 [Leptotrichia sp. oral taxon 218]
ATGAATTTAACATGGACAGATATTGCATGGGGGCAATATGAAGAATGGCAGAAACAGGATAAAAAAATAGTCAAAAAAAT
AAATGAAATAATAAAAGACATCAAAAGGAATGGCAATGAAAGAATAGGAAAGCCTGAGCCTTTAAAGCATGAACTGAGTG
GATACTGGAGCAGAAGAATTACAGATAAGCACAGATTTATATATAAACTGACAGAAACAAGTATAGTAGTTATTGCCTGT
GCAAATCATTATGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11157.93 Da
• Isoelectric Point: 7.2050

>AT163093 WP_003837894.1 NZ_CP056244:c325110-324814 [Citrobacter freundii]
MKDELFADLLASAEEMVRIEKGEETPKPEHVHTFSEIDVKAIREATGLRQQDFAIAVGVSYDLVKSWETKRRQPTGAPRK
LLLLIQKNPFIINQLKAI

Nucleotide

• Length: 297 bp

>AT163093 NZ_CP072377:c1212276-1212019 [Leptotrichia sp. oral taxon 218]
ATGATAGCTACAAATTATTCAAATGTGAGAAATAATTTAAAAAAATATTGTGATAAGGCAACTGATGATTATGAAACAGT
CATTATAACAAGAAAAAACGACAAAAATGTGGTACTGATGAGTGAAGAGGAATATAACAATTTGATGGAAAATCTTTACA
TTATGTCAAATAAGAAATATTATAATGAACTGTTAAAAAGTAAAGCGGAAGCGGAAGCGGGGAATGTACAAATACATGAT
ATAATTGAGGTGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A4U6IRD5