Detailed information of TA system TA162985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4763045..4763805
• Replicon: chromosome
• Accession: NZ_CP056200
• Organism: Citrobacter sp. RHBSTW-00986

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HV345_RS22940
• Protein ID: WP_019077936.1
• Coordinates: 4763045..4763356 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV345_RS22945
• Protein ID: WP_094761751.1
• Coordinates: 4763353..4763805 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV345_RS22910	4758713..4759615	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV345_RS22915	4759612..4760247	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV345_RS22920	4760244..4761173	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV345_RS22925	4761210..4761584	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
HV345_RS22930	4761584..4761826	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HV345_RS22935	4762031..4762960	+	930	WP_181636098.1	alpha/beta hydrolase	-
HV345_RS22940	4763045..4763356	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV345_RS22945	4763353..4763805	+	453	WP_094761751.1	helix-turn-helix domain-containing protein	Antitoxin
HV345_RS22950	4763823..4764764	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV345_RS22955	4764809..4765246	-	438	WP_149382140.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV345_RS22960	4765243..4766115	-	873	WP_181636099.1	virulence factor BrkB family protein	-
HV345_RS22965	4766109..4766708	-	600	WP_181636100.1	glucose-1-phosphatase	-
HV345_RS22970	4766813..4767616	-	804	WP_016155173.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV345_RS22975	4767650..4768546	-	897	WP_019077939.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T162985 WP_019077936.1 NZ_CP056200:4763045-4763356 [Citrobacter sp. RHBSTW-00986]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T162985 NZ_CP072204:3533429-3533647 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16968.36 Da
• Isoelectric Point: 5.9259

>AT162985 WP_094761751.1 NZ_CP056200:4763353-4763805 [Citrobacter sp. RHBSTW-00986]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNEAEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT162985 NZ_CP072204:3533029-3533403 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7L6U900