Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 35700..36349 Replicon chromosome
Accession NZ_CP056200
Organism Citrobacter sp. RHBSTW-00986

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag HV345_RS00180 Protein ID WP_016157800.1
Coordinates 35700..36041 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag HV345_RS00185 Protein ID WP_016157799.1
Coordinates 36050..36349 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV345_RS00165 32136..33464 + 1329 WP_046276191.1 MFS transporter -
HV345_RS00170 33607..34998 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
HV345_RS00175 35147..35599 + 453 WP_181636128.1 DUF1198 family protein -
HV345_RS00180 35700..36041 + 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV345_RS00185 36050..36349 + 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
HV345_RS00190 36468..37652 + 1185 WP_016155040.1 purine ribonucleoside efflux pump NepI -
HV345_RS00195 37708..39090 - 1383 WP_047358473.1 glycoside hydrolase family 1 protein -
HV345_RS00200 39183..39476 - 294 WP_019078023.1 hypothetical protein -
HV345_RS00205 39607..40023 + 417 WP_016157796.1 GNAT family N-acetyltransferase -
HV345_RS00210 40195..41013 + 819 WP_016157795.1 lipoprotein NlpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T162969 WP_016157800.1 NZ_CP056200:35700-36041 [Citrobacter sp. RHBSTW-00986]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

>T162969 NZ_CP072203:c3752382-3752050 [Gordonia polyisoprenivorans]
GTGATTCGCGGCGAAATCTGGACCGTTGCGGGTGGTGTGTATGCATCCAAGCCGCGGCCGGCCGTCATCGTGCAGGATGA
CCTGTTCGATTCAACTCTGTCGGTGGTAGTTGCTCCGATCACGAGCCAGCTCATCGATGCGCCCCTGTTGCGGATTCGTA
TCCCGGGTGATCGCGATTCGATCTCGGGCCTCGAGCGGGACAGTGACGTGATGGTTGACAAGCTAACCGCGGTCCGACGG
TCGAATGTTCAGACGCGTGTCGGTCGTCTGACATCCGACCAACTCGTTGAAGTCGAGCGCTCGTTGATGGCGTTTCTGGG
ACTAGCGCGATGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT162969 WP_016157799.1 NZ_CP056200:36050-36349 [Citrobacter sp. RHBSTW-00986]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp

>AT162969 NZ_CP072203:c3752534-3752379 [Gordonia polyisoprenivorans]
ATGCGCCCGTTGCAGGTCTGGGTTCCTGACGTACGAACGCCAGAGTTTGCCGCGCAAGCCCACAGGCAATCGGTTCTCGT
GGCAGAGGCAGACGCCGATGGGGATGAGCAGGATTTCGTCGAGGCTGTCGCCGCGCCTTGGGACGACGAGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References