Detailed information of TA system TA162967

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3003538..3004174
• Replicon: chromosome
• Accession: NZ_CP056199
• Organism: Enterobacter sp. RHBSTW-00994

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6CLM4
• Locus tag: HV346_RS14535
• Protein ID: WP_001607612.1
• Coordinates: 3003845..3004174 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV346_RS14530
• Protein ID: WP_001553123.1
• Coordinates: 3003538..3003840 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV346_RS14510	2998716..2999633	-	918	WP_181620013.1	nitrogen assimilation transcriptional regulator NAC	-
HV346_RS14520	3000192..3001454	+	1263	WP_181620014.1	integrase arm-type DNA-binding domain-containing protein	-
HV346_RS14525	3002146..3003033	-	888	WP_181623794.1	integrase domain-containing protein	-
HV346_RS14530	3003538..3003840	-	303	WP_001553123.1	XRE family transcriptional regulator	Antitoxin
HV346_RS14535	3003845..3004174	-	330	WP_001607612.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV346_RS14540	3004695..3004916	-	222	WP_046077354.1	helix-turn-helix domain-containing protein	-
HV346_RS14545	3004913..3005380	-	468	WP_181620015.1	hypothetical protein	-
HV346_RS14550	3005754..3007709	+	1956	WP_181620016.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2980271..3004916	24645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (5-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12529.28 Da
• Isoelectric Point: 8.9627

>T162967 WP_001607612.1 NZ_CP056199:c3004174-3003845 [Enterobacter sp. RHBSTW-00994]
MFDHWFTSLNDTDRASVLAALLVLREKGPGLSRPYADTIRGSRYSNMKELRIQSRGDPIRAFFAFDPARTGIVLCAGNKV
GNEKRFYDEMLPIADREFTNWLNTLKEKE

Nucleotide

• Length: 330 bp

>T162967 NZ_CP072203:c691936-691646 [Gordonia polyisoprenivorans]
ATGAGCGAGGACCCCGGCTGCTCGCCCGACCCGTACCGGGTCGAGGTCGCATCCCCAGCCCGTCGCGACCTCGAGCGACT
CCCCTCCCGGATCGTCCACGCCGTCATCGAGTTCATCTCCGGCCCCCTTGCCGAAAACCCCCACCGGCTCGGCAAGCCGT
TGCGCGACCAACTCGAAGGCCTCCGTAGTGCCCGCCGCGGCGACTATCGCATCCTGCTCCGCATCGACGATTCCGGCCAC
ACGATCATCATCGTCAGAATCGACCACCGCGCTCACGCCTACCGCGCCTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 5.1786

>AT162967 WP_001553123.1 NZ_CP056199:c3003840-3003538 [Enterobacter sp. RHBSTW-00994]
MGRTLEQLIADEKPEVVDEAQAMATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT162967 NZ_CP072203:c692208-691933 [Gordonia polyisoprenivorans]
ATGACGACAGTGCCACTCGGTGAAGCCAAGGACAAGCTCTCGGCGCTCATCGACAGCGCCGCGACCACGCACGACATCAT
CACGATCACCAAACACGGCAAACCGGCTGCGGTGCTCATGTCGGCCGACGACCTCGAATCGCTGCACGAGACGATCTACT
GGCTCTCGAGGTCCGGGACCCGCGACGACATGGCTACTGCCGACGCCGAGTACTCGGCCGGCCACACGACTTCTCTCGAG
GATTTGCGCGCCGAACACGGCATGCCGCCCGAATGA

Structures

Toxin

• AlphaFold DB: A0A7L6CLM4