Detailed information of TA system TA162943

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3786244..3786841
• Replicon: chromosome
• Accession: NZ_CP056191
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A9J6S5A1
• Locus tag: HV347_RS18570
• Protein ID: WP_004893639.1
• Coordinates: 3786524..3786841 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: R4YH91
• Locus tag: HV347_RS18565
• Protein ID: WP_004142561.1
• Coordinates: 3786244..3786531 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS18535	3782324..3782572	+	249	WP_032410665.1	DUF1158 domain-containing protein	-
HV347_RS18540	3782590..3782931	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HV347_RS18545	3782962..3784077	-	1116	WP_020316605.1	MBL fold metallo-hydrolase	-
HV347_RS18550	3784257..3784838	+	582	WP_032410661.1	TetR/AcrR family transcriptional regulator	-
HV347_RS18555	3784838..3785206	+	369	WP_032421096.1	MmcQ/YjbR family DNA-binding protein	-
HV347_RS18560	3785326..3785979	+	654	WP_004178896.1	oxygen-insensitive NAD(P)H nitroreductase	-
HV347_RS18565	3786244..3786531	-	288	WP_004142561.1	helix-turn-helix transcriptional regulator	Antitoxin
HV347_RS18570	3786524..3786841	-	318	WP_004893639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV347_RS18575	3787026..3788069	-	1044	WP_032410657.1	DUF2157 domain-containing protein	-
HV347_RS18580	3788735..3789601	-	867	WP_004151823.1	helix-turn-helix transcriptional regulator	-
HV347_RS18585	3789710..3791137	+	1428	WP_009308097.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-88)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12100.35 Da
• Isoelectric Point: 11.2767

>T162943 WP_004893639.1 NZ_CP056191:c3786841-3786524 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPSIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T162943 NZ_CP072199:c702199-701777 [Serratia marcescens subsp. marcescens ATCC 13880]
GTGGCCCAGTGGCGATGTGATATCCGCATTTCCTGGCGCACCCAGCTGTTTTCACTGTTGACCCACGGCGTTCTGATCCT
GCTGATCCTGATTTCGCCCTGGCCGGAGGGCTTTGGCCCGCTCTGGCTGGTGCTGCTGACGCTGGTGGTGTTTCAGTGCA
TTCGCAGCCAGAAGCGCATCGCCGCGGTGCAGGGTGAGCTGCGGCTGCTGGCGGACAGGCGTTTCAGCTGGCACGGCCGC
GAGTGGCGGCTGGCGAAAAAACCGTGGATGCCGGGTTACGGCATGCTGTTGACGCTGCAGCCGATGGAGGGCAAAAAGCG
CCGCCGGCTGTGGCTGGCTTCCGACTGCATGAGCAAAGAGGAGTGGCGCCACCTGCGGCAGCTGTTGCTGCATCCGCCGG
CGGGTAACGGCGAGGAACCCTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10771.46 Da
• Isoelectric Point: 10.2212

>AT162943 WP_004142561.1 NZ_CP056191:c3786531-3786244 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Nucleotide

• Length: 288 bp

>AT162943 NZ_CP072199:c702446-702180 [Serratia marcescens subsp. marcescens ATCC 13880]
ATGGATATTAACAACAAAGCACGTATTCACTGGGCTTGCCGTCGTGGCATGCGCGAGCTGGACATCTCCATCATGCCGTT
CTTCGAATACGAATACGACAGCCTGAATGACGCGGACAAGGCGCTGTTTATCCGCCTGCTGGAGTGTGACGATCCCGATT
TGTTCAACTGGCTGATGAATCATGGCGCGCCGCAGGATGGCGAGCTGCAGAGAATGGTGACCCTGATCCAAACGCGAAAT
AAAGACCGTGGCCCAGTGGCGATGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0S3DIQ1