Detailed information of TA system TA162931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 368651..369297
• Replicon: chromosome
• Accession: NZ_CP056191
• Organism: Klebsiella pneumoniae strain RHBSTW-00995

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV347_RS01755
• Protein ID: WP_023317328.1
• Coordinates: 368651..368998 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV347_RS01760
• Protein ID: WP_032412314.1
• Coordinates: 368998..369297 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV347_RS01745	364577..366010	+	1434	WP_002920564.1	glycogen synthase GlgA	-
HV347_RS01750	366028..368475	+	2448	WP_002920561.1	glycogen phosphorylase	-
HV347_RS01755	368651..368998	+	348	WP_023317328.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV347_RS01760	368998..369297	+	300	WP_032412314.1	XRE family transcriptional regulator	Antitoxin
HV347_RS01765	369360..370868	-	1509	WP_032412313.1	glycerol-3-phosphate dehydrogenase	-
HV347_RS01770	371073..371402	+	330	WP_002920552.1	thiosulfate sulfurtransferase GlpE	-
HV347_RS01775	371453..372283	+	831	WP_004151408.1	rhomboid family intramembrane serine protease GlpG	-
HV347_RS01780	372333..373091	+	759	WP_002920548.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• HigA2-like, helix-turn-helix domain (9-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13562.60 Da
• Isoelectric Point: 6.2327

>T162931 WP_023317328.1 NZ_CP056191:368651-368998 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
ILCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T162931 NZ_CP072172:3737809-3738174 [Rhizobium sp. AB2/73]
ATGATCCTGGTCGATACGTCGATCTGGATCGATCACTTTCATACGCCGGAGGCGCGGCTTCAATCTCTGCTGGAGCAAAA
ACAAGTCGTCGTTCATCCCTTTGTCGTTGGTGAGATCAGCCTTGGCAATCTGCGGCAATATGATCTCGTGATAGGAAGCC
TCCGTAGACTGCCTGCAATTGCGAGAGCCTCGGATGAGGACGTTCTTTATTTCATCCGAAGCAACAAACTGCAGGGTACG
GGAATCGGCTATATCGATGCCCATCTCGCGGCGGCCACGATACTGACGCCCGGAACCTATCTCTGGACGCGTGACAGGCG
GCTGCAACGAGTCGCCGAAAACCTCGGAATTGCCGCGCCGCTATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10828.64 Da
• Isoelectric Point: 10.1464

>AT162931 WP_032412314.1 NZ_CP056191:368998-369297 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGIKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT162931 NZ_CP072172:3737615-3737812 [Rhizobium sp. AB2/73]
ATGCGCACTACCGTAGCCATCGATGACAAGTTGCTTGCGGATGCACGCGAGGTTACCGGTCTCGCCGAGACCTCGGCAAT
CCTACGTGAAGCATTGAAAGCCCTTGTGGAACGGGAAGCCGCCAAAACGCTGATACGTCTCGGCGGCACCGAACCGAATA
TCACGGCTCCCCCTCGTCGTCAGTTTGGCGTCGAATGA