Detailed information of TA system TA162913

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2102165..2102825
• Replicon: chromosome
• Accession: NZ_CP056185
• Organism: Citrobacter sp. RHBSTW-01013

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2I8S535
• Locus tag: HV350_RS10120
• Protein ID: WP_053390179.1
• Coordinates: 2102165..2102518 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV350_RS10125
• Protein ID: WP_053390178.1
• Coordinates: 2102523..2102825 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV350_RS10105	2098660..2100615	-	1956	WP_181630409.1	hypothetical protein	-
HV350_RS10110	2100981..2101448	+	468	WP_181630410.1	hypothetical protein	-
HV350_RS10115	2101445..2101666	+	222	WP_053390180.1	helix-turn-helix domain-containing protein	-
HV350_RS10120	2102165..2102518	+	354	WP_053390179.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV350_RS10125	2102523..2102825	+	303	WP_053390178.1	XRE family transcriptional regulator	Antitoxin
HV350_RS10130	2103324..2104211	+	888	WP_181630412.1	integrase domain-containing protein	-
HV350_RS10135	2104741..2106003	-	1263	WP_181630413.1	integrase arm-type DNA-binding domain-containing protein	-
HV350_RS10140	2106567..2107430	+	864	WP_181630416.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-81)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13442.36 Da
• Isoelectric Point: 9.0134

>T162913 WP_053390179.1 NZ_CP056185:2102165-2102518 [Citrobacter sp. RHBSTW-01013]
VWTIKTTDMFDHWFSSLNDIDRASVLAALLVLREKGPGLSRPYADTLRGSRFSNMKELRIQSRGDPIRAFFAFDPARTGI
VLCAGNKVGNEKRFYDEMIPVADREFTNWLNTIKEKE

Nucleotide

• Length: 354 bp

>T162913 NZ_CP072167:c338294-338028 [Agrobacterium larrymoorei]
ATATTGCTCAGCTGGACGTCCAAGGGCTGGGAAGATTACCTATATTGGCAGCAGGCGGACAAAAAAGTTCTCGTCCGAAT
TAATGAGCTGATCCGGGATGCCTTACGGCACCCGTTCGAGGGTATAGGAAAGCCAGAGCCCTTACGTTTCAACCTCAAAG
GACTCTGGTCCCGTCGTATCACGCAAGAGCACCGACTTATTTATGCAATTCGGGATGAGGGCGATGGCAGAACGCTCGTT
GTGCTCCAATGTCGCTATCACTATTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11040.86 Da
• Isoelectric Point: 6.4738

>AT162913 WP_053390178.1 NZ_CP056185:2102523-2102825 [Citrobacter sp. RHBSTW-01013]
MGRTLEQLIADEKPEVVAEAQAMATDILLNIHLAELREKVQKTQVEMAKALGIRQPTVAGMEKPGRDLKLSTLKRYVEAT
GGKLRLDVELPDGSHYGFVL

Nucleotide

• Length: 303 bp

>AT162913 NZ_CP072167:c338624-338373 [Agrobacterium larrymoorei]
ATGCGTACTGTAATGTTCTCGACTGCAAGAAGTGAGCTTTCCACGCTGCTCGATCAGGTCTCGCACGACCGCGTTGCAGT
GGAAATCGTTCGCAGAGACAAGCCCTCAGCCATCCTCATCGATAAGAACGAATATGAGGGATTGATGGAGACGCTTCATC
TATATTCTTCCCCCGCCAACATCGGTCGTTTGATGGAAGCGATGGATGAGATGGATAAAGGCGACTATCAACAGTCGGAT
CTCATCGATTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S535