Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 35636..36285 Replicon chromosome
Accession NZ_CP056185
Organism Citrobacter sp. RHBSTW-01013

Toxin (Protein)


Gene name higB Uniprot ID A0A7L6TVT2
Locus tag HV350_RS00175 Protein ID WP_016157800.1
Coordinates 35636..35977 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A7L6TWH1
Locus tag HV350_RS00180 Protein ID WP_016157799.1
Coordinates 35986..36285 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV350_RS00160 32072..33400 + 1329 WP_181629547.1 MFS transporter -
HV350_RS00165 33543..34934 + 1392 WP_003023929.1 hexose-6-phosphate:phosphate antiporter -
HV350_RS00170 35083..35535 + 453 WP_181629548.1 DUF1198 domain-containing protein -
HV350_RS00175 35636..35977 + 342 WP_016157800.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV350_RS00180 35986..36285 + 300 WP_016157799.1 helix-turn-helix transcriptional regulator Antitoxin
HV350_RS00185 36404..37588 + 1185 WP_016155040.1 purine ribonucleoside efflux pump NepI -
HV350_RS00190 37644..39026 - 1383 WP_047498768.1 glycoside hydrolase family 1 protein -
HV350_RS00195 39119..39412 - 294 WP_019078023.1 hypothetical protein -
HV350_RS00200 39543..39959 + 417 WP_016155037.1 GNAT family N-acetyltransferase -
HV350_RS00205 40131..40949 + 819 WP_181629549.1 lipoprotein NlpA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(8-88)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13061.92 Da        Isoelectric Point: 5.6550

>T162908 WP_016157800.1 NZ_CP056185:35636-35977 [Citrobacter sp. RHBSTW-01013]
MWEVETTDVFDKWFDVQTEALKEDMLAAMMILSEYGPQLGRPFADTVNASAFSNMKELRVQHQGNPIRAFFVFDPSRHGI
VLCAGDKTGLNEKKFYKEMIRLADAEYRNHLNK

Download         Length: 342 bp

>T162908 NZ_CP072163:c586111-585683 [Rhizobium sp. B21/90]
ATGATCCTGCTCGATACCAACGTCATTTCCGAGCCGCTCAAGTCGCAACCCGATCCACGGGTGGCCGCATGGATCGATAC
GCAGGCCGTCGAAACGCTCTACGTCGCTGCGATCTCACTCGCGGAATTGCGCTACGGCATCGCCGTGCATCCTGACGGCA
AGAAGAAACAAGCCGTGCAGGAAAAGCTTGATCGGTTGATGCTGCCTTTGTTCCGGGGCCGCATCATGCCGTTCGATGAA
GCCGCCGCCCAGGCTTATGCCGAGCTGCGGGCCGCAGCGGCGAAGAAGGGCAAGGCGATCGGCGATTCAGACGGCTATAT
CGCTGCCACGGCCAAGGCGAATGGTCTGATGATCGCCACCCGCGACACCAGCCCTTTCGAGGCCGCCGGCCTGACTGTCA
TCAATCCGTGGGAGGCATCTAATGGCTGA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10988.84 Da        Isoelectric Point: 8.6007

>AT162908 WP_016157799.1 NZ_CP056185:35986-36285 [Citrobacter sp. RHBSTW-01013]
MATLKELMAKQSPESQERIAEKVEVLRQVIALNMLREELNLSQAELASAMGVKQPTIAKMEQADNDPRLSTLKRYVTALG
GELSIDVKLPTGKRVAFHL

Download         Length: 300 bp

>AT162908 NZ_CP072163:c586347-586108 [Rhizobium sp. B21/90]
ATGGCATCTATCACGATCCGAAACGTGCCCGACGAAGTGCATCGGGCGCTTCGGGTGCGCGCTGCTCAGCACGGTCGCAG
CGCCGAGGCGGAAATTCGCATCATCCTGGAACAGGCCGCCAAGCCGGAAGGCCGAGTCAAACTCGGCAGCCTGCTCACAC
AAATAGGCCAGGAAGTCGGCGGCTTCGATCTGACGATCGAGCGAGACCGCACCCCGTCGCCGCCGGTGAGCTTTGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L6TVT2

Antitoxin

Source ID Structure
AlphaFold DB A0A7L6TWH1

References