Detailed information of TA system TA162907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4770735..4771495
• Replicon: chromosome
• Accession: NZ_CP056180
• Organism: Citrobacter sp. RHBSTW-01044

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HV352_RS22740
• Protein ID: WP_019077936.1
• Coordinates: 4770735..4771046 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV352_RS22745
• Protein ID: WP_104651900.1
• Coordinates: 4771043..4771495 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV352_RS22710	4766403..4767305	+	903	WP_003028691.1	formate dehydrogenase O subunit beta	-
HV352_RS22715	4767302..4767937	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV352_RS22720	4767934..4768863	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV352_RS22725	4768900..4769274	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
HV352_RS22730	4769274..4769516	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HV352_RS22735	4769721..4770650	+	930	WP_181635100.1	alpha/beta hydrolase	-
HV352_RS22740	4770735..4771046	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV352_RS22745	4771043..4771495	+	453	WP_104651900.1	helix-turn-helix domain-containing protein	Antitoxin
HV352_RS22750	4771513..4772454	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV352_RS22755	4772499..4772936	-	438	WP_016157874.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV352_RS22760	4772933..4773805	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV352_RS22765	4773799..4774398	-	600	WP_049282314.1	glucose-1-phosphatase	-
HV352_RS22770	4774503..4775306	-	804	WP_016155173.1	DeoR/GlpR family DNA-binding transcription regulator	-
HV352_RS22775	4775340..4776236	-	897	WP_104651901.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T162907 WP_019077936.1 NZ_CP056180:4770735-4771046 [Citrobacter sp. RHBSTW-01044]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T162907 NZ_CP072163:c265193-264315 [Rhizobium sp. B21/90]
ATGGCTAAAACACCAGCGAACATGGCGAAGTCGGTCAAGGACCGGCTTCTCAATATAGCCCGCAAAGAAAGTCGCGCCTT
CGACGTCTTGCTGGTGCGGTTCGCGCTCGAACGGCTGCTGTATCGCCTGTCAATCTCAGCACATCGGGACCGCTTCGTCC
TGAAAGGCGGTATGTTGGTAACGGTTTGGGTGGCCGACGATAATCGGGTGACGCGGGATGCGGACTTTCTCGGACATGGA
GACGCAGATCCTGCCCGCCTGATCGCCGACTTCGGTGAGATCATGGCGATTGAGAGCGACGACGGACTCGCCTTCGACAT
TGATGCGCTCGCTGCGACAACCATTCGAGAAGAAATGGAATATGGCGGCGTTCGTCTCAGGACTGCAGCATATCTCGAAA
GGACCCGCATCTCCGTTACAATCGACATTGGCTTCGGAGACGCTATGGCCGATGCGACGCAACGGCTCGACTATCCAGCC
CTGCTGGATTTTCCCGCTCCGCAGGTTCGTTCCTATCCGCCCGCTACGGTGATCGCCGAGAAATTCCAGGCGATGGTGGC
GCTTGGCGTCCTTAACGGGCGCATGAAGGACTATTATGACCTGTGGGCGATACCGCGCGCGATCGACATTCCCGCTGATG
ATTTGGATGCCGCCATCCGCGTGACGTTCGAACGCCGTCGGACCCCAATCCCTAGAGAACGTCCACCAGGCCTCTCGGTC
GAGATGTTTAGCGACGAAGGGAAGCAGCGGCAATGGCGTGCTTATGCTGCATCGCTCGAACTAGAAGCTGTTTCGCTGGA
ATCGATCATTGGACGGGTTTGGGAGTTGATCGGCCCGTCATGCGCGAGGATCGTAGCAAGAAACGACAATAAGACATGA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 17028.41 Da
• Isoelectric Point: 5.9259

>AT162907 WP_104651900.1 NZ_CP056180:4771043-4771495 [Citrobacter sp. RHBSTW-01044]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVTLLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT162907 NZ_CP072163:c265806-265186 [Rhizobium sp. B21/90]
ATGCCATCGGCGCAAGATTCGCAAAAATTGAAGCTCAGAAGCCTGCTCAATGAGCGCAGCATGATGCATGCGCATGAACT
GCGCGAGGCTGGTATTAGCGCGCAAACAATCGCGCGCGCCGTCGAAGGCGGTGAAATCGATCGTATCTCGCGCGGCGTTT
ATCAGAAGCGTGGCGCAGAAGTCGAAGAGAACCAGATCCTTGCTGAAGCCATCATGCGTGCGCCCAAAGGCGTCATCGCT
CTGGTCTCGGCGCTTGCCTTCCACGGACTAACCGACCAAATGCCGCGGCGCGTTTGGATAGCGATCGGGACGACCGATTG
GGCGCCCGTACAGTCCTATCCCCCGCTGCGTATGGTCCGCTTCACGGAACCATATCTGCGTCAGGGCATCGAACATCAAG
TAATCGCAGGCATTGACGTTCCGGTCTATTCGATCCCGAAAACCCTTGCTGACATGTTCCGAAACCCCAAACTGGTCGAT
CGATCGGTCGCCGTCGAAGGCCTGCGCGCTGCTCTTCAGCAACGCAAGGCAACTCCAAGCGCAATCGCCCAGGCCGCGAA
GGCCGGCGGCGCCTGGAAAGTCATGCAACCCTATCTTGAGGCGCTCACCTCCAATGGCTAA

Structures

Toxin

• AlphaFold DB: A0A7L6U900