Detailed information of TA system TA162885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3503729..3504378
• Replicon: chromosome
• Accession: NZ_CP056168
• Organism: Enterobacter roggenkampii strain RHBSTW-00695

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S2AEP5
• Locus tag: HV268_RS17130
• Protein ID: WP_045416590.1
• Coordinates: 3503729..3504091 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV268_RS17135
• Protein ID: WP_123922794.1
• Coordinates: 3504079..3504378 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV268_RS17110	3499064..3500035	-	972	WP_123922791.1	LacI family transcriptional regulator	-
HV268_RS17115	3500111..3501253	-	1143	WP_181530671.1	Gfo/Idh/MocA family oxidoreductase	-
HV268_RS17120	3501246..3502271	-	1026	WP_181530672.1	sugar phosphate isomerase/epimerase	-
HV268_RS17125	3502287..3503522	-	1236	WP_181530674.1	MFS transporter	-
HV268_RS17130	3503729..3504091	+	363	WP_045416590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV268_RS17135	3504079..3504378	+	300	WP_123922794.1	helix-turn-helix transcriptional regulator	Antitoxin
HV268_RS17140	3504489..3505877	-	1389	WP_023343530.1	phenylalanine transporter	-
HV268_RS17145	3505981..3507954	-	1974	WP_123922795.1	PhoX family phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-80)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13908.76 Da
• Isoelectric Point: 5.1960

>T162885 WP_045416590.1 NZ_CP056168:3503729-3504091 [Enterobacter roggenkampii]
MWNVETTDRFDDWFFEQTTALKEDVLATMHILAEFGPTLGRPYVDTVKDSAYANMKELRIQHAGNPIRAFFAFDPDRKAI
VLCAGDKTGCNQKRFYNEMIALADVEYSRHLADKEAIWQR

Nucleotide

• Length: 363 bp

>T162885 NZ_CP072156:1048763-1049092 [Rhizobium sp. B230/85]
GTGGATCGCGGAGACATCTGGCACGTCGATCTGGAACCGATCCGTGGACGTGAACAGGCCGGCGCGCGGTATGTGCTGGT
GGTTACCAAGAAAAGCTTCAATGCCATCGGCACGCCGATAGTGGTGCCTATAACGACCGGCGGTGAGTTTGCCCGTTTCA
AAGGATTTGCCGTGTCGTTGTCCGGCGCGGGAACGCGGAGTTCGGGTGTGATCATGTGCAATCAGTTGCGGTCGCTCGAC
CTTAGGGCGCGCGGCGGGAAATACGTGGAAAGTGTGCCCGATTTCATCATGTCCGAAGTTCTCGCCAGGATCAGTACGTT
CTTCGACTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10888.66 Da
• Isoelectric Point: 9.8627

>AT162885 WP_123922794.1 NZ_CP056168:3504079-3504378 [Enterobacter roggenkampii]
MATLKELIAQQSAESQERIAAKVEVMRRIVALNQLREELNISQTELAAAMGVKQPTLAKIEQPGNDPRLSTLKRYVSALG
GEMSIDVTLPNGKRIAFQI

Nucleotide

• Length: 300 bp

>AT162885 NZ_CP072156:1048506-1048763 [Rhizobium sp. B230/85]
ATGGCGACATCGACACTTCGCAATGTGGGAGGCTCTGTCATGATGACGATCCCGAAAGCTGTCGTTGATGAGTTGGGACT
GACAGCCAATACCAAGGTCGAGGTATTGGCAGAGGATGGGCGCGTCGTTGCGGTTCCGCACAAGCGGCCGAAGTACACGC
TCGATGAGCTTTTGGCGCAGTGCGATCTTGATGCACCGTTCACAGAGGAAGATCGGCAATGGCTCGATGATGCACCGTCT
GGCCGAGAGGTCATCTAG

Structures

Toxin

• AlphaFold DB: A0A1S2AEP5