Detailed information of TA system TA162869

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85747..86001
• Replicon: plasmid pRHBSTW-00777_2
• Accession: NZ_CP056166
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HV276_RS23500
• Protein ID: WP_001312851.1
• Coordinates: 85852..86001 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	85747..85805 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS23470	81876..82097	+	222	WP_000412081.1	hypothetical protein	-
HV276_RS23475	82105..82854	+	750	WP_181503373.1	type-F conjugative transfer system pilin acetylase TraX	-
HV276_RS23480	82928..83488	+	561	WP_000139377.1	fertility inhibition protein FinO	-
HV276_RS23485	83642..83836	-	195	WP_181503374.1	hypothetical protein	-
HV276_RS23490	84205..84804	+	600	WP_181503375.1	hypothetical protein	-
HV276_RS23495	84973..85203	+	231	WP_001736714.1	hypothetical protein	-
-	85747..85805	-	59	NuclAT_0	-	Antitoxin
-	85747..85805	-	59	NuclAT_0	-	Antitoxin
-	85747..85805	-	59	NuclAT_0	-	Antitoxin
-	85747..85805	-	59	NuclAT_0	-	Antitoxin
HV276_RS23500	85852..86001	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
HV276_RS23505	86316..86606	+	291	WP_001558271.1	DUF1778 domain-containing protein	-
HV276_RS23510	86597..87079	+	483	WP_000405249.1	GNAT family N-acetyltransferase	-
HV276_RS23515	87364..87624	+	261	WP_000078108.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..87925	87925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T162869 WP_001312851.1 NZ_CP056166:85852-86001 [Escherichia marmotae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T162869 NZ_CP072147:608060-609322 [Rhizobium sp. K1/93]
ATGACGGTAAGGGTCCTTAACGTCTGGTGGAATGGTCGCATTGTCGGACAGTTCACCCAAGATCAGCACGGCGACATTGG
CTTCGCCTACGCCGAAGCTTGGCTTGAGGACGAAAAGGCACTTCCGCTTTCTGTCTCCCTACCGAAGCGTCCAGAGCGTT
TCTCTCGCCGTGAATGCCGCCCATTTTTTGGCGGCCTACTGCCAGAGGAAAGCCAGCGTCTGGTTGCGGCGCAGGCGTTG
GGTGTTTCACCTGCCAACGACTTCGCCCTTCTTGATCGCTTGGGCGGCGACGTCGCCGGCGCGCTTCAACTTTTGCCCGA
AGACCAGGAACCGATTGGAGGGGGCCTGATTGCCGATCGGCAAACAGCAGCTCTCGATGAGGTCGGAATCGTCCGGATAC
TGGACGCACTTCCGACGAGGCCGCTCTTGGCCGGCCAGGAAGGCTTGAGGCTATCGCTTGCGGGCGCCCAATCAAAAGCT
CCAGTTGTTCTCATCGATGGCCAAGTCGCACTTCCCTTACCAGGCCAGGCGACGACGCACATCTTGAAGCCACCGATTGC
GCGCTTTCCAGGGACGACTGAGAATGAAGCCTTCGCGATGAGGCTTGCTGCCGCAATCGGGCTCGACGTTGCTCCCGTCG
ACCCGAGGTCCGCGAATGGTCGCCCTTTCCTTTTGGTCAACAGATACGATCGCCACCACGATGGCGACGGTGTGGTTCTC
CGCATCCATCAGGAAGACTTCTGCCAAGCGCTGGGTGTGCCACCTGAAACAAAGTATGCAAGCGAGGGGGGGCCGACCTT
CAAGGATTGCTTCGAGCTGTTGCGTCGGGTCTCTGCGCGGCCGGGCACGGACATCGCTAAGCTTTTGGACGCTGCGATTT
TCAATCTCGTCATCGGAAATGCGGATGCTCACGGTAAGAATTTCTCGATCCTCTATGACCATTCTGGACCTAGGATGGCT
CCGCTGTACGACCTGCTTTCCACCGTGGCCTATCCAGATCTTTCCCCTAAATTGGCTATGCGGATCGGCAAACGCGCGAC
GCTTGCTGACATGGACGCTGATGGCTGGCAGGCTTTCTCGAAGGAGACAGGTGTCAGCTTACCTTTAGTGCGCCGTCGGG
TGAAAGAACTGACTGATGCCACAGGGGACGCGATCCCCCGGTTATTGGAAGATATGTCCCCACTTGCCGCAGACCCTGTG
TCGTTAAATCGCGTAGCGGCTTTGATCGCAGACCGTGCCAAACTCGTGGCCCTGAGCATTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT162869 NZ_CP056166:c85805-85747 [Escherichia marmotae]
AGATACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin