Detailed information of TA system TA162864

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2495283..2495654
• Replicon: chromosome
• Accession: NZ_CP056165
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: F4VC37
• Locus tag: HV276_RS11920
• Protein ID: WP_001317028.1
• Coordinates: 2495283..2495477 (+)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2495476..2495654 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS11895	2490981..2491202	+	222	WP_000560230.1	killing protein KilR	-
HV276_RS11900	2491196..2491372	+	177	WP_000245522.1	hypothetical protein	-
HV276_RS11905	2491447..2491722	+	276	WP_001516463.1	phage protein	-
HV276_RS11910	2491824..2494424	+	2601	WP_115763388.1	exodeoxyribonuclease VIII	-
HV276_RS11915	2494417..2495226	+	810	WP_000166319.1	recombination protein RecT	-
HV276_RS11920	2495283..2495477	+	195	WP_001317028.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HV276_RS11925	2495470..2495658	+	189	WP_001302840.1	DUF1187 family protein	-
-	2495476..2495654	-	179	NuclAT_0	-	Antitoxin
-	2495476..2495654	-	179	NuclAT_0	-	Antitoxin
-	2495476..2495654	-	179	NuclAT_0	-	Antitoxin
-	2495476..2495654	-	179	NuclAT_0	-	Antitoxin
HV276_RS11930	2495758..2495973	+	216	WP_000079604.1	excisionase XisR	-
HV276_RS11935	2495975..2497210	+	1236	WP_001516465.1	site-specific integrase	-
HV276_RS11940	2497262..2498197	+	936	WP_001516466.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
HV276_RS11945	2498292..2499665	-	1374	WP_105224583.1	ATP-dependent RNA helicase DbpA	-
HV276_RS11950	2499695..2499868	-	174	WP_001296046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2452419..2497210	44791

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7018.89 Da
• Isoelectric Point: 8.9538

>T162864 WP_001317028.1 NZ_CP056165:2495283-2495477 [Escherichia marmotae]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T162864 NZ_CP072146:c1519985-1519692 [Rhizobium sp. K1/93]
ATGCCCGAAGTTCGGCAGACGATCTTGTTCAGGCGTTGGTTCGATGGCCTGAAAGACAAGCGTGCGGTTGAGCGGATCAC
CCAGCGTATCGTTCGACTGCAAGCCGGTTTGTTTGGAGACATAAAATTCTTCGATGGCATCGGCGAGTTGCGCGTCGATC
ATGGGCCTGGGTATCGGGTTTATTTTGTGCAGCGCGGATCTGTCCTGGTCATTTTACTGTGGGGCGGCGACAAGAGTTCT
CAAACCCGCGATATACGGAAAGCCAAGGCCATGGCGAATGATTTGGAGATTTGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT162864 NZ_CP056165:c2495654-2495476 [Escherichia marmotae]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCCGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A7L6LHV7

Antitoxin