Detailed information of TA system TA162857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2017170..2017518
• Replicon: chromosome
• Accession: NZ_CP056165
• Organism: Escherichia marmotae strain RHBSTW-00777

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: -
• Locus tag: HV276_RS09465
• Protein ID: WP_181503340.1
• Coordinates: 2017276..2017518 (-)
• Length: 81 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2017170..2017332 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV276_RS09425	2012613..2013269	-	657	WP_000122137.1	carbon-nitrogen hydrolase family protein	-
HV276_RS09430	2013370..2013600	-	231	WP_000916764.1	DNA polymerase III subunit theta	-
HV276_RS09435	2013739..2014113	+	375	WP_181503283.1	CopC domain-containing protein YobA	-
HV276_RS09440	2014117..2014989	+	873	WP_000879335.1	copper homeostasis membrane protein CopD	-
HV276_RS09445	2015002..2015343	+	342	WP_000976465.1	YebY family protein	-
HV276_RS09450	2015736..2016812	-	1077	WP_001189094.1	phage integrase Arm DNA-binding domain-containing protein	-
HV276_RS09455	2016778..2017059	-	282	WP_001311878.1	excisionase	-
HV276_RS09460	2017166..2017330	-	165	Protein_1842	double-strand break reduction protein RcbA	-
-	2017170..2017332	+	163	NuclAT_1	-	Antitoxin
-	2017170..2017332	+	163	NuclAT_1	-	Antitoxin
-	2017170..2017332	+	163	NuclAT_1	-	Antitoxin
-	2017170..2017332	+	163	NuclAT_1	-	Antitoxin
HV276_RS09465	2017276..2017518	-	243	WP_181503340.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
HV276_RS09470	2017551..2018231	-	681	WP_181503284.1	YqaJ viral recombinase family protein	-
HV276_RS09475	2018228..2019013	-	786	WP_181503285.1	phage recombination protein Bet	-
HV276_RS09480	2019019..2019315	-	297	WP_181503341.1	host-nuclease inhibitor protein Gam	-
HV276_RS09485	2019293..2021320	-	2028	WP_181503286.1	exonuclease	-
HV276_RS09490	2021344..2021640	-	297	WP_000022247.1	hypothetical protein	-
HV276_RS09495	2021715..2021891	-	177	WP_000245534.1	hypothetical protein	-
HV276_RS09500	2021885..2022106	-	222	WP_000560220.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1995280..2079622	84342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 81 a.a.
• Molecular weight: 8701.98 Da
• Isoelectric Point: 10.3674

>T162857 WP_181503340.1 NZ_CP056165:c2017518-2017276 [Escherichia marmotae]
VRYTKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVLKRKVALLLTGQGTQKQS

Nucleotide

• Length: 243 bp

>T162857 NZ_CP072143:c76501-76313 [Rhizobium sp. L51/94]
ATGAAGAGCGTCGATATTATTTCTGCCTTGACGAATGATGGATGGTTTGAGGTTGCCAAGAAAGGCAGCCATGTCCAGCT
TAAACACCCGACAAAGCGAGGCCGTGTAACCGTTCCTCATCCTAAGCGAGATATCCCGATGGGGACGCTCAAGAGCATCG
AAAAGCAATCTGGGCTAAAACTGAGGTAA

Antitoxin

Nucleotide

• Length: 163 bp

>AT162857 NZ_CP056165:2017170-2017332 [Escherichia marmotae]
GAGGACAGAAGTTTCTCGCAATTAAAATTTATCAGCTTTACTTTCTGCTCTCTGGACACGCCTGCTTCTTTTTTCCCTGA
GAGCATTTTTTCGCATTCCGATTTGGTTAACTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAA

Structures

Antitoxin