Detailed information of TA system TA162733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2876277..2876937
• Replicon: chromosome
• Accession: NZ_CP056132
• Organism: Enterobacter hormaechei strain RHBSTW-00916

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV326_RS13860
• Protein ID: WP_047053529.1
• Coordinates: 2876584..2876937 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0Q4Z7
• Locus tag: HV326_RS13855
• Protein ID: WP_017383313.1
• Coordinates: 2876277..2876579 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV326_RS13840	2872447..2873772	+	1326	WP_047053533.1	SidA/IucD/PvdA family monooxygenase	-
HV326_RS13845	2873799..2875988	+	2190	WP_047053531.1	TonB-dependent siderophore receptor	-
HV326_RS13855	2876277..2876579	-	303	WP_017383313.1	helix-turn-helix transcriptional regulator	Antitoxin
HV326_RS13860	2876584..2876937	-	354	WP_047053529.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV326_RS13865	2877128..2878078	-	951	WP_003859532.1	HTH-type transcriptional regulator Cbl	-
HV326_RS13870	2878175..2879092	-	918	WP_003859531.1	nitrogen assimilation transcriptional regulator NAC	-
HV326_RS13880	2879581..2880504	-	924	WP_047053526.1	L,D-transpeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13522.40 Da
• Isoelectric Point: 10.1729

>T162733 WP_047053529.1 NZ_CP056132:c2876937-2876584 [Enterobacter hormaechei]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFNPNRTGI
VLCAGNKIGNERRFYDEMLLVADREYTRWLNTLKERN

Nucleotide

• Length: 354 bp

>T162733 NZ_CP071988:831390-831599 [Staphylococcus epidermidis]
ATGAAATTGAAAAAAGGATTGATAAATGTTTCAGGTATTAAAACAACTGAACAAGCTAAACAGCTCAAAGATCATTTATC
TAAGATGATAGGCATCAATAGTGTGGATATCGATTACCAAATGAATGAGATTCGAGTAGAATTTGACACTCCTGCAAATT
TAAATAACATTGAAAAAGAAATTTACGATTACGGATTTCGTATTTTGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11028.85 Da
• Isoelectric Point: 6.4727

>AT162733 WP_017383313.1 NZ_CP056132:c2876579-2876277 [Enterobacter hormaechei]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT162733 NZ_CP071988:831090-831383 [Staphylococcus epidermidis]
ATGGCAGACATGGAGAGTGCACATCACTCAGAGCAAATAAAAACAAATTTAAAATCAAGATTAAATAGAATAGAAGGTCA
AGTAAGAGCAATAAATCGTATGATTGATGATGACGTTTACTGTGATGATGTACTAACTCAAATTAGAGCAACACGTTCAG
CATTGAATAGTGTAGCTACTAAATTACTAGACCATCACATGAAAGGTTGTATTATGGAAAAAATAAACGACGGTGCGCAA
GAAGAAGCAATGGATGAATTGCTCGTGACATTCCAAAAATTAATAAAAGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0Q4Z7