Detailed information of TA system TA162716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 92368..93014
• Replicon: chromosome
• Accession: NZ_CP056132
• Organism: Enterobacter hormaechei strain RHBSTW-00916

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV326_RS00475
• Protein ID: WP_047050720.1
• Coordinates: 92664..93014 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7H8UAV4
• Locus tag: HV326_RS00470
• Protein ID: WP_045335986.1
• Coordinates: 92368..92667 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV326_RS00445	87836..88420	-	585	WP_023299002.1	type 1 fimbrial protein	-
HV326_RS00450	88473..89075	-	603	WP_047050717.1	response regulator transcription factor	-
HV326_RS00455	89910..90698	+	789	WP_045329485.1	winged helix-turn-helix domain-containing protein	-
HV326_RS00460	90700..91179	+	480	WP_047050718.1	hypothetical protein	-
HV326_RS00465	91422..92303	+	882	WP_047050719.1	helix-turn-helix domain-containing protein	-
HV326_RS00470	92368..92667	-	300	WP_045335986.1	XRE family transcriptional regulator	Antitoxin
HV326_RS00475	92664..93014	-	351	WP_047050720.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV326_RS00480	93287..93539	-	253	Protein_95	DNA-binding protein	-
HV326_RS00485	93642..93983	-	342	WP_006812446.1	SymE family type I addiction module toxin	-
HV326_RS00490	94120..94296	+	177	WP_071962261.1	toxin-antitoxin system HicB family antitoxin	-
HV326_RS00495	94336..95892	-	1557	WP_047050722.1	hypothetical protein	-
HV326_RS00500	96187..96507	-	321	WP_059443702.1	TA system toxin CbtA family protein	-
HV326_RS00505	96528..96854	-	327	WP_059443453.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV326_RS00510	96867..97337	-	471	WP_015703840.1	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	89910..111142	21232

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13497.59 Da
• Isoelectric Point: 8.8416

>T162716 WP_047050720.1 NZ_CP056132:c93014-92664 [Enterobacter hormaechei]
MWTVLFSKVFEQWLLEQEDGLQEKVLADLLNLQNYGPRLPRPYADTVKGSQYKNMKELRIQYAGRPVRAFFAFDPVRQAI
VLCAGDKRNDKTFYEKLIRIADAEFTLHLTSLEAAK

Nucleotide

• Length: 351 bp

>T162716 NZ_CP071974:2139865-2140125 [Trueperella pecoris]
GTGAGCTATCGCGTTGAACTAACAACAGCCGCTGCTCGCCAGATTAAGAAACTGCCTCACGAAGCACGCGGTCGCATTTT
GGACGCGCTAGAGAAATTGGCGGACGATCCTCGGCCCCGCGGCGCAAAGAAGCTTGTCGGCGAACAGAATGCATGGCGAA
TTCGCCTCGGAGATTACCGAATTATTTATGACATCTTTGACGACATACTGACTGTAACAATCGTTCGCGCTGGGCATCGT
CGCGAAATTTACCGCAGCTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11129.87 Da
• Isoelectric Point: 6.4714

>AT162716 WP_045335986.1 NZ_CP056132:c92667-92368 [Enterobacter hormaechei]
VKTLNEVIASRSPESQQRIQEMAEEMFLETGLQLLREELQLSQKHVADLMGVSQPAVTQLEQRGNDLKIATLKRYIEAMG
GKLSINVELPTGKRVAFHL

Nucleotide

• Length: 300 bp

>AT162716 NZ_CP071974:2139605-2139868 [Trueperella pecoris]
ATGAACACGATCACCAGCGAGGTCTCGACTCGCGAACTACGCAGCCAGCTCTCAGACGTTCTTGGGCGGGCCATGTACGC
CGGCGAACGCATCGGCGTCACCCGTCACGGAAAGCTAGCAGCTGTCGTCGTGAGCGTCGCAGATCTTGAGGCGCTCGAGG
AATTCGAGATGGCTCAGGATGTAGCCGCCTACCGTGCCGCTCGCGCGGAAGACGATGGCGGACGGGTGAGCCTTGATGAA
CTACGACGCACATTGACACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7H8UAV4