Detailed information of TA system TA162691

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2825545..2826205
• Replicon: chromosome
• Accession: NZ_CP056126
• Organism: Enterobacter asburiae strain RHBSTW-01009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376FK36
• Locus tag: HV349_RS13895
• Protein ID: WP_029741461.1
• Coordinates: 2825852..2826205 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV349_RS13890
• Protein ID: WP_054830246.1
• Coordinates: 2825545..2825847 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV349_RS13865	2821337..2821519	+	183	Protein_2716	DUF4102 domain-containing protein	-
HV349_RS13870	2821589..2822569	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
HV349_RS13875	2822698..2823549	-	852	Protein_2718	hypothetical protein	-
HV349_RS13880	2824006..2824407	-	402	WP_054830247.1	RidA family protein	-
HV349_RS13885	2824549..2825469	+	921	WP_059346999.1	LysR family transcriptional regulator	-
HV349_RS13890	2825545..2825847	-	303	WP_054830246.1	XRE family transcriptional regulator	Antitoxin
HV349_RS13895	2825852..2826205	-	354	WP_029741461.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV349_RS13900	2826383..2827276	-	894	WP_181599400.1	LysR family transcriptional regulator	-
HV349_RS13905	2827447..2828055	+	609	WP_081051431.1	glutathione S-transferase family protein	-
HV349_RS13910	2828099..2829049	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
HV349_RS13915	2829146..2830063	-	918	WP_008499475.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2821589..2822569	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (25-89)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13531.36 Da
• Isoelectric Point: 9.0310

>T162691 WP_029741461.1 NZ_CP056126:c2826205-2825852 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLEDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLNTLKERE

Nucleotide

• Length: 354 bp

>T162691 NZ_CP071954:c5024855-5024544 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11024.80 Da
• Isoelectric Point: 5.7316

>AT162691 WP_054830246.1 NZ_CP056126:c2825847-2825545 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKAGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT162691 NZ_CP071954:c5024543-5024253 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A376FK36