Detailed information of TA system TA162680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 31604..32334
• Replicon: chromosome
• Accession: NZ_CP056126
• Organism: Enterobacter asburiae strain RHBSTW-01009

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A376FEI5
• Locus tag: HV349_RS00155
• Protein ID: WP_029741423.1
• Coordinates: 31604..31918 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV349_RS00160
• Protein ID: WP_167347079.1
• Coordinates: 31915..32334 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV349_RS00130	27270..28358	+	1089	WP_059347371.1	cellulase family glycosylhydrolase	-
HV349_RS00135	28261..29445	-	1185	WP_029741426.1	multidrug efflux MFS transporter EmrD	-
HV349_RS00140	29621..30454	-	834	WP_181599606.1	DMT family transporter	-
HV349_RS00145	30517..30963	-	447	WP_059347369.1	GNAT family N-acetyltransferase	-
HV349_RS00150	31028..31117	-	90	WP_023309830.1	type I toxin-antitoxin system toxin TisB	-
HV349_RS00155	31604..31918	+	315	WP_029741423.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV349_RS00160	31915..32334	+	420	WP_167347079.1	helix-turn-helix domain-containing protein	Antitoxin
HV349_RS00165	32482..32580	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
HV349_RS00170	32687..34375	+	1689	WP_059347368.1	acetolactate synthase large subunit	-
HV349_RS00175	34379..34666	+	288	WP_010426528.1	acetolactate synthase small subunit	-
HV349_RS00180	34767..35360	+	594	WP_029741420.1	transcriptional regulator UhpA	-
HV349_RS00185	35357..36862	+	1506	WP_059347367.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12361.46 Da
• Isoelectric Point: 10.2219

>T162680 WP_029741423.1 NZ_CP056126:31604-31918 [Enterobacter asburiae]
MHLISMKAILDAVTQFPQHKDELLFLGRTIEKSHCPTPAALKKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T162680 NZ_CP056126:31604-31918 [Enterobacter asburiae]
ATGCATTTAATCTCTATGAAAGCTATTTTGGATGCGGTAACCCAGTTTCCGCAGCACAAGGATGAACTCCTTTTTTTGGG
TCGCACCATCGAAAAGAGCCATTGCCCAACACCTGCTGCTCTGAAAAAACTGTTTCCGACGCTGGATAACTTTAAGTATC
TGGATAAGCATTATGTTATTGATATTGCAAACAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15487.99 Da
• Isoelectric Point: 4.5348

>AT162680 WP_167347079.1 NZ_CP056126:31915-32334 [Enterobacter asburiae]
MMIVADAMKATHALVAAVPLLGEQPSEKDYEDALELVEYLLMNEPDSPLLDIVCARISRYEANRPDIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFEDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT162680 NZ_CP056126:31915-32334 [Enterobacter asburiae]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCATGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATGAGGATGCGCTAGAGCTGGTTGAGTATCTTCTTATGAACGAGCCCGATAGCCCCTTGTTGGATATTGTGT
GCGCCAGGATTAGCCGTTACGAAGCCAATCGGCCAGATATTGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGT
ATTGCGGTTTTAAGAACCTTGATGGATCAGTACAACCTGACGATATCGGATTTTGAGGATGAAATTGGCAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACTCTGAACCATATTAAAAAGCTGGCGGCGAGGTTTGGGGTATCAC
CTGCGCTGTTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A376FEI5