Detailed information of TA system TA162652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1646538..1647140
• Replicon: chromosome
• Accession: NZ_CP056118
• Organism: Enterobacter sp. DSM 30060

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: HWQ15_RS07795
• Protein ID: WP_022649837.1
• Coordinates: 1646829..1647140 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWQ15_RS07790
• Protein ID: WP_032626927.1
• Coordinates: 1646538..1646828 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWQ15_RS07775	1644036..1644938	+	903	WP_063149405.1	formate dehydrogenase subunit beta	-
HWQ15_RS07780	1644935..1645570	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
HWQ15_RS07785	1645567..1646496	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
HWQ15_RS07790	1646538..1646828	-	291	WP_032626927.1	helix-turn-helix domain-containing protein	Antitoxin
HWQ15_RS07795	1646829..1647140	-	312	WP_022649837.1	hypothetical protein	Toxin
HWQ15_RS07800	1647290..1648231	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
HWQ15_RS07805	1648276..1648713	-	438	WP_063146849.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HWQ15_RS07810	1648710..1649591	-	882	WP_023298923.1	virulence factor BrkB family protein	-
HWQ15_RS07815	1649585..1650184	-	600	WP_003861947.1	glucose-1-phosphatase	-
HWQ15_RS07820	1650303..1651103	-	801	WP_023298924.1	DeoR/GlpR family DNA-binding transcription regulator	-
HWQ15_RS07825	1651138..1652034	-	897	WP_063149406.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T162652 WP_022649837.1 NZ_CP056118:c1647140-1646829 [Enterobacter sp. DSM 30060]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T162652 NZ_CP071931:543949-544290 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10685.18 Da
• Isoelectric Point: 10.5888

>AT162652 WP_032626927.1 NZ_CP056118:c1646828-1646538 [Enterobacter sp. DSM 30060]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALSQ

Nucleotide

• Length: 291 bp

>AT162652 NZ_CP071931:543719-543949 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Toxin

• AlphaFold DB: A0A155DUZ6