Detailed information of TA system TA162634

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2958642..2959244
• Replicon: chromosome
• Accession: NZ_CP056114
• Organism: Escherichia coli strain Ec394-330266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HWD38_RS14280
• Protein ID: WP_000897302.1
• Coordinates: 2958642..2958953 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HWD38_RS14285
• Protein ID: WP_000356397.1
• Coordinates: 2958954..2959244 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWD38_RS14255 (2954556)	2954556..2955155	+	600	WP_001296610.1	glucose-1-phosphatase	-
HWD38_RS14260 (2955149)	2955149..2956021	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HWD38_RS14265 (2956018)	2956018..2956455	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
HWD38_RS14270 (2956500)	2956500..2957441	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HWD38_RS14275 (2957505)	2957505..2958413	-	909	WP_001385591.1	alpha/beta hydrolase	-
HWD38_RS14280 (2958642)	2958642..2958953	+	312	WP_000897302.1	hypothetical protein	Toxin
HWD38_RS14285 (2958954)	2958954..2959244	+	291	WP_000356397.1	NadS family protein	Antitoxin
HWD38_RS14290 (2959603)	2959603..2959881	+	279	WP_001296612.1	hypothetical protein	-
HWD38_RS14295 (2960278)	2960278..2960496	+	219	WP_001251293.1	CopG family transcriptional regulator	-
HWD38_RS14300 (2960681)	2960681..2961421	-	741	WP_000608806.1	hypothetical protein	-
HWD38_RS14305 (2961446)	2961446..2962294	-	849	WP_001038650.1	hypothetical protein	-
HWD38_RS14310 (2962584)	2962584..2962826	+	243	WP_001068514.1	CopG family transcriptional regulator	-
HWD38_RS14315 (2963008)	2963008..2963937	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T162634 WP_000897302.1 NZ_CP056114:2958642-2958953 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T162634 NZ_CP071899:1246754-1247068 [Pseudomonas stutzeri]
ATGATCTTTATCGAGACACCGATCTTCACCAAGCGCCTGCGGGATCTGCTTAGCGATGACAGCTACGCGGAGTTTCAGCG
CCAACTGGCTGACCGGCCGGACATGGGCGATGTGATTGAAGGTACTGGCGGCATTCGCAAGGTCCGCGTTGCGTCTAGCG
GTCACGGCAAGCGAGGCGGGTCCAGGGTCATCTACTACCACTTCACGGCGGCTTCGCAGATCGCATTGCTGCTGATCTAT
CCGAAGAACGAGAAGGACGACCTGACGGCAGACGAGCGCAAGGTGCTCAAGCAAATCATCGAACGGTGGAGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT162634 WP_000356397.1 NZ_CP056114:2958954-2959244 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT162634 NZ_CP071899:1247074-1247364 [Pseudomonas stutzeri]
ATGAGCAAATTCTTCGAAGACCTTCTGGAAAGCGTCCAGCAGATGGACGAGATTCACCGTGGCGAACGTCAACCCTCGCG
GGAGTTCGTCGTCGACTCGCTGCAGGTGAAAGAGATCCGCAAGGCGACCGGGCTGACCCAGGCCAAGTTCGCGGCGATGA
TCGACGTTCAACTGGGTACGCTGCGTAATTGGGAACAAGGTCGACGGGAGCCGACCGGACCGGCAAAGGCGCTGCTGCGC
GCCATCCATAACGATCCGAAGCATGTCATCCAGGCGCTTTCTTCCGTCTGA

Structures

Toxin

• AlphaFold DB: S1P416