Detailed information of TA system TA162626

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 788318..788820
• Replicon: chromosome
• Accession: NZ_CP056114
• Organism: Escherichia coli strain Ec394-330266

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: HWD38_RS04035
• Protein ID: WP_000767819.1
• Coordinates: 788318..788572 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: HWD38_RS04040
• Protein ID: WP_001259253.1
• Coordinates: 788569..788820 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HWD38_RS04010 (784136)	784136..784594	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
HWD38_RS04015 (784811)	784811..786169	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
HWD38_RS04020 (786159)	786159..786221	-	63	WP_010723108.1	membrane protein YoeI	-
HWD38_RS04025 (786436)	786436..787365	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
HWD38_RS04030 (787411)	787411..788235	-	825	WP_000754737.1	SDR family oxidoreductase	-
HWD38_RS04035 (788318)	788318..788572	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
HWD38_RS04040 (788569)	788569..788820	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
HWD38_RS04045 (789103)	789103..789153	+	51	WP_001364200.1	his operon leader peptide	-
HWD38_RS04050 (789299)	789299..790198	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
HWD38_RS04055 (790204)	790204..791508	+	1305	WP_001296211.1	histidinol dehydrogenase	-
HWD38_RS04060 (791505)	791505..792575	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
HWD38_RS04065 (792575)	792575..793642	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	784136..784594	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T162626 WP_000767819.1 NZ_CP056114:c788572-788318 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T162626 NZ_CP071889:c2785930-2785637 [Halomonas sp. XH26]
ATGATCGTAAATTTCAAAGACAAAGCGGTAGAAGACTTATTCAATGGCGAGCATACCAAGGCTGCTCGCAAGCTATGTCC
TCTATCTCTATGGAAAATTGCATCACGAAAACTCGATCTACTGGATTCGGCACAAAAGCTGGATGAACTGAGGGTACCGC
CTGGTAATCGGCTTGAAGCGTTATCAGGCGACAGAGCCGGACAGCATAGTATTCGCATCAATGGACAGTATCGTCTCTGT
TTTAGGTGGACAGAGGCTGGGCCTGATGAGGTCGAGTTAGTCGATTACCACTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT162626 WP_001259253.1 NZ_CP056114:c788820-788569 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT162626 NZ_CP071889:c2785613-2785305 [Halomonas sp. XH26]
ATGATTCGTGTTCCCACACATCGCGAACCGACGCACCCCGGCGAAATGTTACTGGAAGAGTTTTTGCAGCCCATGGACAT
TACCCAGCGTGAGCTGGCCGCTGCTATCCACGTTCCTTACCAGCGGATCAATGAGCTGGTGAATAAAAAACGGGGTGTTA
CGCCCAGCACGGCACTAAGGCTGGCACGATTTTTTGATGTTTCAGCAGATTTTTGGCTGAATTTACAAATGCGCTGGGAT
CTCTTCAAAGCAGAGCAGCTAGAGCACGAGGATCTCTCTAGTATCCAGCACTGGCAGAAGATGGCGTAG

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3