Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4459201..4459763 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HED12_RS21840 Protein ID WP_000605675.1
Coordinates 4459201..4459479 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HED12_RS21845 Protein ID WP_000781370.1
Coordinates 4459479..4459763 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS21815 4454787..4455218 - 432 WP_000152305.1 peroxiredoxin OsmC -
HED12_RS21820 4455563..4455778 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HED12_RS21825 4455880..4456017 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HED12_RS21830 4456174..4457871 + 1698 WP_000433464.1 malate dehydrogenase -
HED12_RS21835 4458005..4459015 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HED12_RS21840 4459201..4459479 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HED12_RS21845 4459479..4459763 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HED12_RS21850 4459992..4460645 - 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HED12_RS21855 4460638..4461522 - 885 WP_001240581.1 formate dehydrogenase N subunit beta -
HED12_RS21860 4461535..4464582 - 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T162595 WP_000605675.1 NZ_CP056077:4459201-4459479 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T162595 NZ_CP071870:c3360049-3359783 [Propioniciclava sp. MC1595]
ATGAGCGAAGCGCCTCTCCCGGTTGCGTGGACCCCGACCGCCAAGCGCGCCCTCACCAGGCTGCCGGAGAAGGCAGCCGC
CGCGGCCGTGGAGTTCATCTACGGACCGGTCGCTGAGATCCCCCGTCGGGTAGGAAAGGCGCTCCGCCGCGAACTCGAAG
GCCTCTACAGCGCACGCCGCGGCGCCTTCCGGATCATCTACCGGATCTCCGACGTCGTCACCATCATGGCCATCGACCAC
CGAGCCGACACCTACCGGAGCCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT162595 WP_000781370.1 NZ_CP056077:4459479-4459763 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT162595 NZ_CP071870:c3360327-3360046 [Propioniciclava sp. MC1595]
ATGACTGTTGAGGGGACGATGGCGCTGAGGGACGTCAAGAACCACCTGTCCGAGGTCGTGGACCAGGTGGAACGCGAGCA
CGACCGGGTCGTGATCACCAAGCACGGCCGCCCCGCAGCGGTCGTGGTTAGCGTCGACGACCTCGCCTCGCTGGAGGAGA
CCCTTGACATCGCCGGCCGTCCCCAGTTGATGCGGCAGATCCGAGCGAGCCTGACCGAGATCGCCGCCGGCGAGGCAGAG
GTGGTCACCAAGGACGAGATCCTGCGCACGCTCAGCACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References