Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3219523..3220250 Replicon chromosome
Accession NZ_CP056077
Organism Escherichia coli strain MS1170

Toxin (Protein)


Gene name higB Uniprot ID Q3YYE6
Locus tag HED12_RS15915 Protein ID WP_000547563.1
Coordinates 3219523..3219834 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HED12_RS15920 Protein ID WP_000126293.1
Coordinates 3219831..3220250 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HED12_RS15885 3214664..3216373 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HED12_RS15890 3216383..3216925 + 543 WP_085455500.1 formate hydrogenlyase subunit HycF -
HED12_RS15895 3216925..3217692 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HED12_RS15900 3217689..3218099 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HED12_RS15905 3218092..3218562 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HED12_RS15910 3218605..3219360 + 756 WP_202791683.1 hypothetical protein -
HED12_RS15915 3219523..3219834 + 312 WP_000547563.1 type II toxin-antitoxin system HigB family toxin Toxin
HED12_RS15920 3219831..3220250 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HED12_RS15925 3220342..3220770 - 429 WP_202791684.1 hypothetical protein -
HED12_RS15930 3221155..3221682 + 528 WP_001078777.1 electron transport protein HydN -
HED12_RS15935 3221835..3224087 + 2253 WP_096939072.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12398.11 Da        Isoelectric Point: 9.7105

>T162584 WP_000547563.1 NZ_CP056077:3219523-3219834 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T162584 NZ_CP071861:c4082714-4082340 [Enterobacter cloacae]
GTGTGGACAATTAACACGACGGACAGGTTCGATTGCTGGTTTCGTTCACTTGACGATACTGATCGGGCCAGCGTGCTGGC
AGCACTGCTTGTTTTGCGAGAAAAGGGGCCTGCTTTGTCGAGGCCCTATGCCGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTGCGCATTCAGAGTAAGAGCGATCCGTTGCGGGCATTTTTCGCGTTTGACCCAAACCGTACCGGCATT
GTGCTCTGTGCCGGCAATAAGGTCGGCAACGAAAGACGCTTTTACGATGAAATGCTTCTGGTGGCCGACCGGGAATTCAC
TCACTGGTTGAATACATTAAAAGAAAGGACGTTACGCGATGGGCAGAAACCTTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT162584 WP_000126293.1 NZ_CP056077:3219831-3220250 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT162584 NZ_CP071861:c4082356-4082054 [Enterobacter cloacae]
ATGGGCAGAAACCTTGAGCAGCTTATAGCGGATGAAAAATCGGATGTTGTTGCCAGCGCTCAGGCAATGGCTGCCGACAT
TTTGCTTAATATCCATCTGGCAGAGCTACGTGAAAAGGTGCAAAAAACGCAGGTTGAAATGGCGCAGGCGCTGGGCATCC
GACAGCCCACCGTCGCGGTCATGGAAAAGCCCGGGCGCGATCTGAAACTCTCTTCACTCAAGCGCTACGTTGAGGCTGCG
GGTGGAAAGCTTACGCTTGACGTTGAGTTACCGGATGGTTCGCATTATGAGTTTGTGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QA22

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References