Detailed information of TA system TA162568

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 30018..30287
• Replicon: plasmid pEC50-1
• Accession: NZ_CP056074
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HUS69_RS22810
• Protein ID: WP_001372321.1
• Coordinates: 30162..30287 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	30018..30083 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS22775	25064..25312	+	249	WP_071606928.1	hypothetical protein	-
HUS69_RS22780	25785..26324	+	540	WP_000290791.1	single-stranded DNA-binding protein	-
HUS69_RS22785	26387..26620	+	234	WP_000005990.1	DUF905 family protein	-
HUS69_RS22790	26686..28644	+	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
HUS69_RS22795	28699..29133	+	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
HUS69_RS22800	29130..29892	+	763	Protein_40	plasmid SOS inhibition protein A	-
HUS69_RS22805	29861..30049	-	189	WP_001299721.1	hypothetical protein	-
-	29861..30085	+	225	NuclAT_0	-	-
-	29861..30085	+	225	NuclAT_0	-	-
-	29861..30085	+	225	NuclAT_0	-	-
-	29861..30085	+	225	NuclAT_0	-	-
-	30018..30083	+	66	-	-	Antitoxin
HUS69_RS23535	30071..30220	+	150	Protein_42	plasmid maintenance protein Mok	-
HUS69_RS22810	30162..30287	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HUS69_RS23540	30507..30737	+	231	WP_071587244.1	hypothetical protein	-
HUS69_RS23545	30735..30908	-	174	Protein_45	hypothetical protein	-
HUS69_RS22815	30978..31184	+	207	WP_000547939.1	hypothetical protein	-
HUS69_RS22820	31209..31496	+	288	WP_000107535.1	hypothetical protein	-
HUS69_RS22825	31619..32440	+	822	WP_001234469.1	DUF932 domain-containing protein	-
HUS69_RS22830	32737..33327	-	591	WP_000252680.1	transglycosylase SLT domain-containing protein	-
HUS69_RS22835	33660..34043	+	384	WP_053320747.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HUS69_RS22840	34230..34919	+	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T162568 WP_001372321.1 NZ_CP056074:30162-30287 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T162568 NZ_CP071857:c1481035-1480739 [Vibrio sp. SCSIO 43145]
ATGCAAAAGAACAAATACAAACTGAGTAAATTAGCTCAGACTCATTTACGGAAAATCAAAAGCTATACTGTTAACAACTT
CTCCGAGACCCAGTGGCGAAGCTATAAAGATACGTTACTAACTGGTTTTCAAATGCTTGCTGATAATCCAGCTGTTGGTC
GCAGTTGTGATGACATATACCCAAGCGGGTTCTATTTCCCCGCGGGCAAGCATACCGCTTACTTTACAAAAGAAGATTGC
TTCATTTTAGTTGTAGCCGTACTCGGACAATCACAACTTCCTCAGAACCACTTGTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT162568 NZ_CP056074:30018-30083 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin