Detailed information of TA system TA162565

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 3695464..3696005
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: U9XPG0
• Locus tag: HUS69_RS17870
• Protein ID: WP_000615976.1
• Coordinates: 3695727..3696005 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: HUS69_RS17865
• Protein ID: WP_186240651.1
• Coordinates: 3695464..3695724 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS17845 (3691147)	3691147..3691932	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HUS69_RS17850 (3691904)	3691904..3693616	+	1713	Protein_3541	flagellar biosynthesis protein FlhA	-
HUS69_RS17855 (3693832)	3693832..3694329	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-
HUS69_RS17860 (3694505)	3694505..3695278	-	774	WP_015675129.1	C40 family peptidase	-
HUS69_RS17865 (3695464)	3695464..3695724	+	261	WP_186240651.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
HUS69_RS17870 (3695727)	3695727..3696005	+	279	WP_000615976.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
HUS69_RS17875 (3696161)	3696161..3696901	+	741	WP_001225679.1	peptidoglycan meso-diaminopimelic acid protein amidase	-
HUS69_RS17880 (3696872)	3696872..3697639	-	768	WP_000333380.1	class II glutamine amidotransferase	-
HUS69_RS17885 (3697845)	3697845..3698423	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-84)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10800.59 Da
• Isoelectric Point: 10.0702

>T162565 WP_000615976.1 NZ_CP056073:3695727-3696005 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Nucleotide

• Length: 279 bp

>T162565 NZ_CP071857:c1395150-1394872 [Vibrio sp. SCSIO 43145]
ATGATTTTGTGGGAAGAAGAGTCTCTCAACGATCGCGAAAAGATCTTTGAGTTTCTCTACGACTTCAACCCCGAAGCTGC
TGACAAAACCGACGATATTATTGAAGCAAAAGTTGAGAACCTTTTAGAGCAACCTCATATGGGAGTCCAGCGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATTTCGATGATTGTTTCCTACTGGGTTGAAGGTGATATCATTCGAGTTATG
CGCGTGCTACACCAAAAACAAAAATTCCCAACGGACTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9361.79 Da
• Isoelectric Point: 5.8041

>AT162565 WP_186240651.1 NZ_CP056073:3695464-3695724 [Escherichia coli]
MAANAFVRARIDVDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Nucleotide

• Length: 261 bp

>AT162565 NZ_CP071857:c1395431-1395147 [Vibrio sp. SCSIO 43145]
ATGGACACTAGAATTCAATTTCGTGTTGATGAAGAAACGAAGCGACTAGCCCAACAAATGGCTGAAAGCCAAGGCAGAAC
ACTAAGTGATGCTTGTCGTGAACTGACTGAGCAACTGGCTGACCAACAGAGAAAGACTTTATCTCACGACGCATGGTTAA
CTGAACAAATTAACTTAGCTTTTGAGAAGTTTGACTCAGGAAAAGCCACTTTTATTGACCATGAGTCAGCTAAATCTCGT
ATGGACGAGCGTAAAGCTAAAATCCGTAACCGAGGCAAATTATGA

Structures

Toxin

• AlphaFold DB: U9XPG0