Detailed information of TA system TA162564

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3689275..3689969
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HUS69_RS17830
• Protein ID: WP_001263493.1
• Coordinates: 3689275..3689673 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HUS69_RS17835
• Protein ID: WP_000554757.1
• Coordinates: 3689676..3689969 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3684935)	3684935..3685015	-	81	NuclAT_12	-	-
- (3684935)	3684935..3685015	-	81	NuclAT_12	-	-
- (3684935)	3684935..3685015	-	81	NuclAT_12	-	-
- (3684935)	3684935..3685015	-	81	NuclAT_12	-	-
HUS69_RS17800 (3684275)	3684275..3685519	-	1245	WP_000189541.1	esterase FrsA	-
HUS69_RS17805 (3685611)	3685611..3686069	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HUS69_RS17810 (3686330)	3686330..3687787	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HUS69_RS17815 (3687844)	3687844..3688365	-	522	Protein_3534	peptide chain release factor H	-
HUS69_RS17820 (3688364)	3688364..3688567	-	204	Protein_3535	RtcB family protein	-
HUS69_RS17825 (3688813)	3688813..3689265	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HUS69_RS17830 (3689275)	3689275..3689673	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HUS69_RS17835 (3689676)	3689676..3689969	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HUS69_RS17840 (3690021)	3690021..3691076	-	1056	WP_085452615.1	DNA polymerase IV	-
HUS69_RS17845 (3691147)	3691147..3691932	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
HUS69_RS17850 (3691904)	3691904..3693616	+	1713	Protein_3541	flagellar biosynthesis protein FlhA	-
HUS69_RS17855 (3693832)	3693832..3694329	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T162564 WP_001263493.1 NZ_CP056073:c3689673-3689275 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T162564 NZ_CP071857:c1385408-1385112 [Vibrio sp. SCSIO 43145]
ATGCAAAAGAATAAATATAAGCTAAGTAAATTAGCACAGACCCATTTACAAAAAATCAAAAACTATACCGTCAACAATTT
CTCTGAGATGCAATGGCGAAGCTACAAAGATACCTTACTAACAGGGTTTCAAATGCTTGCTGACAACCCGGCTGTTGGTC
GCAGTTGCGATGAGATATACCCAAGTGGTTTTTATTTCCCAGTGGGTAAGCATACCGCTTACTTTACAAAAGAAGATGGC
TTCATTTTAGTTGTTGCTGTACTCGGTCAATCACAACTTCCGCAGAACCACCTGTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT162564 WP_000554757.1 NZ_CP056073:c3689969-3689676 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT162564 NZ_CP071857:c1385657-1385415 [Vibrio sp. SCSIO 43145]
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGTTACTTCTGAGCGCTCAACGTGAGCCAGTGAAAAT
TAGCAAAAATAGCAAAGATGCCGTAGTAGTTATGTCGATTAAGGACTATGAAGAGCTGGAAGCAATGAAAACTGATTACC
TGAGACATTGTTTCGAGTCAGCTAAGCAAGACTTGGTACAAGGCAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6