Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1863813..1864315 Replicon chromosome
Accession NZ_CP056073
Organism Escherichia coli strain EC50

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HUS69_RS08770 Protein ID WP_000767829.1
Coordinates 1864061..1864315 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID -
Locus tag HUS69_RS08765 Protein ID WP_001610372.1
Coordinates 1863813..1864064 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS69_RS08740 (1858991) 1858991..1860058 - 1068 WP_000080067.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HUS69_RS08745 (1860058) 1860058..1861128 - 1071 WP_001099221.1 histidinol-phosphate transaminase -
HUS69_RS08750 (1861125) 1861125..1862429 - 1305 WP_001295420.1 histidinol dehydrogenase -
HUS69_RS08755 (1862435) 1862435..1863334 - 900 WP_000131774.1 ATP phosphoribosyltransferase -
HUS69_RS08760 (1863480) 1863480..1863530 - 51 WP_001364200.1 his operon leader peptide -
HUS69_RS08765 (1863813) 1863813..1864064 + 252 WP_001610372.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HUS69_RS08770 (1864061) 1864061..1864315 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HUS69_RS08775 (1864398) 1864398..1865222 + 825 WP_000754740.1 SDR family oxidoreductase -
HUS69_RS08780 (1865268) 1865268..1866197 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
HUS69_RS08785 (1866412) 1866412..1866474 + 63 WP_010723108.1 membrane protein YoeI -
HUS69_RS08790 (1866464) 1866464..1867822 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HUS69_RS08795 (1868001) 1868001..1869059 + 1059 WP_001759318.1 thiosulfate utilization transporter TsuA/YeeE -
HUS69_RS08800 (1869073) 1869073..1869300 + 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T162550 WP_000767829.1 NZ_CP056073:1864061-1864315 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T162550 NZ_CP071848:2154537-2154836 [Vibrio sp. SCSIO 43136]
ATGAAGCCTTTTCAATTAACTAATCGAGCAAAATTGGATTTAAAAGATATCGCTATCTTCACAGCTCGTAGATGGGGGCG
TGAGCAAAGGAACCTCTATCTGAAGCAGTTTGATGACTCGTTTTGGTTACTTGCTGAAAACCCAGATATCGGAAAAATTT
GTGATGAGATCCGCCAAGGATATCGGAAATTCCCTCAAGGTAGCCATGTGATTTTTTACCGACTTATTGGCAATCAAAAA
ATTGAAATTATTCGTATCTTGCATAAAAGCATGGACGTAAATCCCCTCTTTGCTCGCTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9289.55 Da        Isoelectric Point: 4.7954

>AT162550 WP_001610372.1 NZ_CP056073:1863813-1864064 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLIDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT162550 NZ_CP071848:2154298-2154540 [Vibrio sp. SCSIO 43136]
ATGGCTAAAAATACAAGTATTACTTTGGGTAAACACTTTGATGGCTTTATTGCGGATCAAATCCAAAGTGGTCGCTATAA
TTCTGCAAGTGAAGTCATTCGCTCGGCATTACGTTTACTCGAATCTCAAGAAACCAAGATGCACACCTTGCGCCAGTTGC
TGGTCGAAGGTGAAGAAAGTGGTACTGCCGACTATGATCTTGACTCCTTCATTGAAGAGCTGGATGAAGAACCCAATCGA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure

References