Detailed information of TA system TA162547

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 758382..759075
• Replicon: chromosome
• Accession: NZ_CP056073
• Organism: Escherichia coli strain EC50

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HUS69_RS03645
• Protein ID: WP_000415584.1
• Coordinates: 758382..758678 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HUS69_RS03650
• Protein ID: WP_000650107.1
• Coordinates: 758680..759075 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUS69_RS03610 (753470)	753470..753784	-	315	WP_000958598.1	putative quinol monooxygenase	-
HUS69_RS03615 (753815)	753815..754396	-	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HUS69_RS03620 (754715)	754715..755047	+	333	WP_000917684.1	DUF2645 family protein	-
HUS69_RS03625 (755093)	755093..756442	-	1350	WP_000673402.1	quorum sensing histidine kinase QseC	-
HUS69_RS03630 (756439)	756439..757098	-	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	-
HUS69_RS03635 (757250)	757250..757642	+	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HUS69_RS03640 (757695)	757695..758177	+	483	WP_186240473.1	GyrI-like domain-containing protein	-
HUS69_RS03645 (758382)	758382..758678	+	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HUS69_RS03650 (758680)	758680..759075	+	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HUS69_RS03655 (759208)	759208..760815	+	1608	WP_186240474.1	ABC transporter substrate-binding protein	-
HUS69_RS03660 (760953)	760953..763211	+	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T162547 WP_000415584.1 NZ_CP056073:758382-758678 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T162547 NZ_CP071848:c1230617-1230204 [Vibrio sp. SCSIO 43136]
GTGAAAGATAAGCCGTTAAAAGTTAGGATCTTTAAACATAAAATCTTGATTGAGGCTTTAAGTGAAAATGAACTTGAGGC
TTTGATTAGCGACTTCAAGACATACAAAGAAACTGGTCAAAAGCCTGACACCTTTGGTCGGGACGAAGCTTACGATCACC
CAAATACCCTACCGATACTAAAATCAGAAGAAGTAAGGCACATTCACTTAGCAGCACATGATGCCCCATTCTTGAGTGCC
GTTCAGTTTTATCAAACCAGTGATAAGCACTTAGTTTACTGCCAAGGGTGGGAAGATCCCAAATGCTATCTACTAATTGC
TATTCTAGCCCCTGATGCCCATGAGCAGGCTCGAAACAGGAATATCATGCATAATTTGGGGGTGATTGCTGAGAGGTTTA
GGCAGCAGTTTTGA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT162547 WP_000650107.1 NZ_CP056073:758680-759075 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT162547 NZ_CP071848:c1230907-1230614 [Vibrio sp. SCSIO 43136]
ATGAAAACAAGTACAATCTATGCAGAAAAGGCTGTGTCATCAAGTGAAGCCAGAAAGCACTTCAAGGATTGCTTATCAGG
CGAGCCAGTTGTAGTTCTATCTAACAATGAACCCGCAGGCTACATGATGAGTGCAGACTTTTTCGAAGGTCTGATGAAAG
CGTTAGAAGATAAGAACCCAAACTCAGTAGCTTCATTTAGGCCAATGACGGCACGCTTGGATGCTATTGCACAGCTGAAT
GCCAAAGCATTGGCTGATCTTGATGAAAACGACATAGGTAATTTTCAAGAGTGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65