Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 693809..694536 Replicon chromosome
Accession NZ_CP056073
Organism Escherichia coli strain EC50

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUS69_RS03340 Protein ID WP_000550189.1
Coordinates 693809..694123 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HUS69_RS03345 Protein ID WP_000560255.1
Coordinates 694120..694536 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUS69_RS03320 (689967) 689967..690953 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
HUS69_RS03325 (691032) 691032..691724 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
HUS69_RS03330 (691801) 691801..692304 - 504 WP_001300832.1 M48 family metallopeptidase -
HUS69_RS03335 (692389) 692389..693525 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUS69_RS03340 (693809) 693809..694123 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUS69_RS03345 (694120) 694120..694536 + 417 WP_000560255.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUS69_RS03350 (694581) 694581..696599 - 2019 WP_023281493.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUS69_RS03355 (697025) 697025..699376 - 2352 Protein_668 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T162546 WP_000550189.1 NZ_CP056073:693809-694123 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T162546 NZ_CP071848:1214645-1214947 [Vibrio sp. SCSIO 43136]
ATGGCTCAAATAGTTTGGACCCAGCCCGCATTATCTGACCTCAATGACATCGCAGAGTATGTTGCCATTGAAAATACGCT
TGCGGCTAAGCAGCTTGTTCAGTCCATTTTTAATGCCATTGAGCGGTTAGAAGCATTTCCAGAATCTGGCCGAACACCAC
CAGAGTTACCAACCCTTCCTTATCGTGAAGTTATCGTTTCCCCGTGTCGGGTGTTTTATAAATACGACGGAAATAGTATC
TATATCTTGTTTGTCATGAGGCAAGAGCAAGACTTAAAGCGGTTTATGCTCAGTAAAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14951.41 Da        Isoelectric Point: 4.5577

>AT162546 WP_000560255.1 NZ_CP056073:694120-694536 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNAPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT162546 NZ_CP071848:1214400-1214657 [Vibrio sp. SCSIO 43136]
ATGAAAGTCGAGTTAGTGACTTCATTAAAACGTGAAGCAACAAAAATCTTAGCGGACCTGCATGACTCGAAAGAGCCTGT
GCTGATTACTGAGCATGGTAAACCCTCGGCATACTTGGTGGATGTCGATGACTATGAATCGATGCAAAAACGCTTGGCAA
TTCTCGAAGGTATTTCGAGGGGCGAGCGAGCCATCGCATCTGAGCAATTGGTTAGCCACGATGAAGCTAAGGACCAAATG
TCGAAATGGCTCAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References