162515

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 678538..679265 Replicon chromosome
Accession NZ_CP056036
Organism Escherichia coli strain EC31

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag HUW39_RS03265 Protein ID WP_000550189.1
Coordinates 678538..678852 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID D3GWQ0
Locus tag HUW39_RS03270 Protein ID WP_000560253.1
Coordinates 678849..679265 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUW39_RS03245 (674704) 674704..675690 - 987 WP_001377074.1 Gfo/Idh/MocA family oxidoreductase -
HUW39_RS03250 (675769) 675769..676452 - 684 WP_001183054.1 vancomycin high temperature exclusion protein -
HUW39_RS03255 (676529) 676529..677032 - 504 WP_001309773.1 M48 family metallopeptidase -
HUW39_RS03260 (677117) 677117..678253 + 1137 WP_000018677.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
HUW39_RS03265 (678538) 678538..678852 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
HUW39_RS03270 (678849) 678849..679265 + 417 WP_000560253.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
HUW39_RS03275 (679310) 679310..681328 - 2019 WP_000121438.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
HUW39_RS03280 (681499) 681499..683850 - 2352 WP_000695439.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Antitoxin HigA

(1-138)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T162515 WP_000550189.1 NZ_CP056036:678538-678852 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T162515 NZ_CP071826:1045326-1045733 [Burkholderia anthina]
ATGAAGTTTCTGCTGGATACCAATGCGGTGATCGCCATTCTAAAAGGCGACCCAGCCGTGCTGGCCCGATTGCATAGGCA
TCAACCCGCGGATTTCGGAATGCCCGCGATCGTCGCGCATGAACTCTATTACGGTGCGTACAAAAGTCAGCGAGTGGAAG
CGAATCTCGCACGCGTCGCGGGGCTCCAGTTCGAGGTCGTATCGTTTGACGCGGAAGATGCTCAACATGCCGGTGAAATT
CGTGCGCAACTGACTGCGGCGGGAACACCGATCGGTCCATATGATGCGTTGATCGCGGGGCAGGCGCGAGCGCGGAGACT
TGTACTCGTTACGCATAACACGCGCGAATTCGAGGGGGTCACTGCGCTGGAGGTCGAGGATTGGCTCGCAGGGCCGCGCG
AAAACTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14965.39 Da        Isoelectric Point: 4.4547

>AT162515 WP_000560253.1 NZ_CP056036:678849-679265 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT162515 NZ_CP071826:1045087-1045329 [Burkholderia anthina]
ATGGAGACTGCCAAGATTTTCAGGCATGGCGGATCGCAGGCCGTCCGTTTGCCGAAGGATTTCCGCTTCGACACGACGGA
GGTACGGATTCGTCGGCACGGCGCGTCTGTCATTCTCGAACCCGTGCCGCAAGACTGGGCATGGCTGACGCCGTTGATCG
GCCCGGTCGACGCAGATTTCGAGGCCGCTGCGACGACCGAACCGGCCGCTCAGGAGCGTCCGGGGCTGGACGTCTTCGAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure
AlphaFold DB A0A829IXD3

References