Detailed information of TA system TA162514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 635490..636289
• Replicon: chromosome
• Accession: NZ_CP056036
• Organism: Escherichia coli strain EC31

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: B7NDB6
• Locus tag: HUW39_RS03045
• Protein ID: WP_000347269.1
• Coordinates: 635490..635954 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: V0YUB5
• Locus tag: HUW39_RS03050
• Protein ID: WP_001309780.1
• Coordinates: 635954..636289 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUW39_RS03015 (630491)	630491..630925	-	435	WP_000948818.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
HUW39_RS03020 (630943)	630943..631821	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
HUW39_RS03025 (631811)	631811..632590	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
HUW39_RS03030 (632601)	632601..633074	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HUW39_RS03035 (633097)	633097..634377	-	1281	WP_000681943.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HUW39_RS03040 (634626)	634626..635435	+	810	WP_000072171.1	aga operon transcriptional regulator AgaR	-
HUW39_RS03045 (635490)	635490..635954	-	465	WP_000347269.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HUW39_RS03050 (635954)	635954..636289	-	336	WP_001309780.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HUW39_RS03055 (636438)	636438..638009	-	1572	WP_001273738.1	galactarate dehydratase	-
HUW39_RS03060 (638384)	638384..639718	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
HUW39_RS03065 (639734)	639734..640504	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T162514 WP_000347269.1 NZ_CP056036:c635954-635490 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Nucleotide

• Length: 465 bp

>T162514 NZ_CP071826:c733775-733437 [Burkholderia anthina]
ATGGTTGACATACTGCGATCCATGCCCTACAGTGCCCCAACATTCAGCATCCGGACCACCGACGTCTTCGACGCGTGGTT
CGCCGGCCTGCCCGACCGTGTCGCCAGGCGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATCCCGGCGACT
GGAAATCCGCCGGCTCGCCCATCGTCGAAATGCGTATCGACCACGGGCCCGGCTATCGCGTCTACTACGTGCGGCGCGGA
ACGATATGGGTGATCCTGCTCTGCGGCGGCGCCAAGTCGACGCAACAGGCGGATATCCGCGCCGCGCACGCGATGCTCGC
GCGGCTCGATTTGGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12345.94 Da
• Isoelectric Point: 4.8616

>AT162514 WP_001309780.1 NZ_CP056036:c636289-635954 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT162514 NZ_CP071826:c733433-733134 [Burkholderia anthina]
ATGGACAAGATCAAGACCCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGACGACGACATGGCCGAATACTTCGACGC
CTGTCTGCGGGAAGCCGGCGACGACCCGGCCTTCATCGCGCATGCGCTCGGCGTGATCGCCCGTGCCCGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAATCCGAGCTTCGGCACG
ATCCTGAAGGTCATCAAGGCACTCGGCCTGCAACTGCACGGCACCAAGGCGCACGCGTGA

Structures

Toxin

• AlphaFold DB: A0A829IY86

Antitoxin

• AlphaFold DB: V0YUB5