Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2907689..2908191 Replicon chromosome
Accession NZ_CP055675
Organism Escherichia fergusonii strain RHB28-C13

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HVY52_RS14140 Protein ID WP_000767829.1
Coordinates 2907689..2907943 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HVY52_RS14145 Protein ID WP_001259255.1
Coordinates 2907940..2908191 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HVY52_RS14110 2902704..2902931 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
HVY52_RS14115 2902945..2904003 - 1059 WP_046080827.1 transport protein YeeE -
HVY52_RS14120 2904182..2905540 - 1359 WP_059228986.1 putrescine/proton symporter PlaP -
HVY52_RS14125 2905530..2905592 - 63 WP_010723108.1 membrane protein YoeI -
HVY52_RS14130 2905807..2906736 - 930 WP_000803361.1 LysR family transcriptional regulator -
HVY52_RS14135 2906782..2907606 - 825 WP_000754745.1 SDR family oxidoreductase -
HVY52_RS14140 2907689..2907943 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HVY52_RS14145 2907940..2908191 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HVY52_RS14150 2908475..2908525 + 51 WP_001364200.1 his operon leader peptide -
HVY52_RS14155 2908671..2909570 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
HVY52_RS14160 2909576..2910880 + 1305 WP_015953522.1 histidinol dehydrogenase -
HVY52_RS14165 2910877..2911947 + 1071 WP_000108922.1 histidinol-phosphate transaminase -
HVY52_RS14170 2911947..2913014 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T162470 WP_000767829.1 NZ_CP055675:c2907943-2907689 [Escherichia fergusonii]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T162470 NZ_CP071797:5541039-5541293 [Pseudomonas fluorescens]
ATGAAAATCCAGTTCACGCCAACGGGCTGGGAAGACTACTTATGGTTTCAACAAAACGATAAGGCCGGTCTCAAACGAAT
CAATCTTTTGATCAAAGCGATCCAGCGCCAACCCTTTGAAGGCTTGGGTAAACCGGAGCCGCTCAAGCACAACATGAGCG
GCTTCTGGTCACGGCAGATAACTGCCGAGCATCGCCTGGTCTATGCAATCGTAGACGACGAAATCTGCGTCATAACTTGC
AGATTTCACTACTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT162470 WP_001259255.1 NZ_CP055675:c2908191-2907940 [Escherichia fergusonii]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT162470 NZ_CP071797:5540788-5541042 [Pseudomonas fluorescens]
ATGGACACTATCAACTACACCACAGCTCGTGCGCACTTGGCTGAAACCATGGATCGCGTGAATGAAGACTGCACCCCGCT
TCTGGTAACCCGCCAAAAAGGCGAGCCTGTAGTAATGATGTCTCTGGCCGAATACAACGCGCTGGAAGAAACGGCTTATC
TGCTGCGCTCTCCGGCCAATGCCGAGCGCTTGATCAAATCGATTGGCGAAATGCGCGCTGGAAAAGCCAAAGTCAGGCAA
CTGATTGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References