Detailed information of TA system TA162457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 1212341..1213020
• Replicon: chromosome
• Accession: NZ_CP055675
• Organism: Escherichia fergusonii strain RHB28-C13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B7LJF3
• Locus tag: HVY52_RS05860
• Protein ID: WP_002431667.1
• Coordinates: 1212341..1212643 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HVY52_RS05865
• Protein ID: WP_000806441.1
• Coordinates: 1212679..1213020 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY52_RS05835	1207717..1209369	+	1653	WP_181203208.1	bifunctional UDP-sugar hydrolase/5'-nucleotidase	-
HVY52_RS05840	1209407..1209910	-	504	WP_000667001.1	hypothetical protein	-
HVY52_RS05845	1209907..1210707	-	801	WP_000439795.1	hypothetical protein	-
HVY52_RS05850	1210731..1211210	-	480	WP_181203209.1	Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK	-
HVY52_RS05855	1211414..1212208	-	795	WP_000365145.1	TraB/GumN family protein	-
HVY52_RS05860	1212341..1212643	+	303	WP_002431667.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HVY52_RS05865	1212679..1213020	+	342	WP_000806441.1	HigA family addiction module antidote protein	Antitoxin
HVY52_RS05870	1213134..1215638	-	2505	WP_000083966.1	copper-exporting P-type ATPase CopA	-
HVY52_RS05875	1215945..1217234	+	1290	WP_181203210.1	APC family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11811.37 Da
• Isoelectric Point: 10.1572

>T162457 WP_002431667.1 NZ_CP055675:1212341-1212643 [Escherichia fergusonii]
MAQKNNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELYLDPHNY

Nucleotide

• Length: 303 bp

>T162457 NZ_CP071796:c2075229-2074834 [Ottowia testudinis]
ATGCGCTACATGCTCGGCACGAATGTGGTGAGCCATCTGGTCCGGGCGCACCCCAGCGTGTCCAGACGGGTGGCGTCAAT
GCCGGCCGGCCATTTGTGTATCTCGGCGGTCACGGAGGCTGAATTGCGATATGGCCTGGAAAAGCATCGCGGCGCGCACC
GCTTGCAAAGCCTGATTTACGAATTTCTGGCAAGCATTCCGATCATGCCGTGGCGGCACGAGGAAGCGATTGTGTACGCA
CAGTTTCGCAACAGCCTTGAAAGGCAAGGCCGCAGCCTTGCAGCACACGATGCGCTGATTGCGGCGCACGCCAAATCGCT
GGGCCTGATTCTGGTGTCCAACGATCAGGCGTTCGTGAATCTGGTCGACATGCCGTTTGAGGATTGGACGCACTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13143.01 Da
• Isoelectric Point: 5.2345

>AT162457 WP_000806441.1 NZ_CP055675:1212679-1213020 [Escherichia fergusonii]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVADYLARREERAKNVA

Nucleotide

• Length: 342 bp

>AT162457 NZ_CP071796:c2075492-2075229 [Ottowia testudinis]
ATGATGCAGACCGCCAAGCTGTTCACCAATGGCCGCAGCCAAGCCGTTCGATTGCCCCGCGAATTTCGTTTTGAAGAAAC
CGAGGTGTACATCCGACGTGACGCGAGCACGGGAGACGTGATCCTGTCCCGCAAGCCTGCGTCGCTGGATCGTTTTTTTG
AGTTGCTCCGTGAAGGCGTGGATGTGCCCCAGGATTTTTTGAGCCCGGACGAGCGGCACGTGGCTGACGATTTGCGCGAC
CCGTTTGCAGGCTGGCAAGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7K4HYG8