Detailed information of TA system TA162452

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 560125..560924
• Replicon: chromosome
• Accession: NZ_CP055675
• Organism: Escherichia fergusonii strain RHB28-C13

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: HVY52_RS02630
• Protein ID: WP_000347270.1
• Coordinates: 560125..560589 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: HVY52_RS02635
• Protein ID: WP_104919918.1
• Coordinates: 560589..560924 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HVY52_RS02600	555126..555560	-	435	WP_181203483.1	PTS sugar transporter subunit IIA	-
HVY52_RS02605	555578..556456	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HVY52_RS02610	556446..557225	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
HVY52_RS02615	557236..557709	-	474	WP_032303829.1	PTS N-acetylgalactosamine transporter subunit IIB	-
HVY52_RS02620	557732..559012	-	1281	WP_181203097.1	tagatose-bisphosphate aldolase subunit KbaZ	-
HVY52_RS02625	559261..560070	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
HVY52_RS02630	560125..560589	-	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
HVY52_RS02635	560589..560924	-	336	WP_104919918.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
HVY52_RS02640	561073..562644	-	1572	WP_181203098.1	galactarate dehydratase	-
HVY52_RS02645	563115..563885	+	771	WP_001058567.1	2-dehydro-3-deoxyglucarate aldolase	-
HVY52_RS02650	563910..564800	+	891	WP_000178100.1	2-hydroxy-3-oxopropionate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T162452 WP_000347270.1 NZ_CP055675:c560589-560125 [Escherichia fergusonii]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T162452 NZ_CP071795:1027366-1027665 [Polaribacter batillariae]
ATGGAATATAAAATCAGTCAAGAGGCAAATCGTGATATCGAAAACATTTGGCTCTACACTTTCGAAAACTGGTCAGCGGA
ACAAGCCGACAGGTATTTTAATCTGATTTTGGACGAAATTGAATATATCGCTAAAAATCCCACTTCAGGAAAAGATTACA
ATGAAATTAGAAAAGGATATTATAGATCTAGAATCAAATCACATTTTATTTTCTACAAAATCAATCGAAAAAAAGAAAGA
GTCGAAATCATTAGAATATTACACCAAAGAATGGATATTGAATCAAGACTTGATGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12349.93 Da
• Isoelectric Point: 4.5392

>AT162452 WP_104919918.1 NZ_CP055675:c560924-560589 [Escherichia fergusonii]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGRKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT162452 NZ_CP071795:1027118-1027366 [Polaribacter batillariae]
ATGCCGAAAAACACTTCTATTCTTCTTGGTGATTACTTCGATAAATTCATCAGTCAACAAATAAAAAGCGGAAAATACTC
TTCCGCAAGTGAAGTCGTTAGAGCCGCTTTGAGAATGTTTGAACATCAGGAATCAAAGAAATCTGAATTGATCAAAGCAC
TCAAAAAAGGTGAAAAATCCGGATTTGTAGAAAACTTAGACCGAACTAAACTCTTGAAAGAAATTCATCAAAAGTATTTG
ACTGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90