Detailed information of TA system TA162450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 23988..24634
• Replicon: plasmid pRHB41-C23_4
• Accession: NZ_CP055602
• Organism: Escherichia fergusonii strain RHB41-C23

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3P1YQM6
• Locus tag: HV014_RS23370
• Protein ID: WP_025667955.1
• Coordinates: 24287..24634 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV014_RS23365
• Protein ID: WP_001673379.1
• Coordinates: 23988..24287 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV014_RS23330	19204..19452	+	249	WP_000730008.1	hypothetical protein	-
HV014_RS23335	19515..19664	+	150	WP_000003577.1	hypothetical protein	-
HV014_RS23340	20479..20940	+	462	Protein_34	ash family protein	-
HV014_RS23345	20937..22079	+	1143	WP_181199445.1	ORF6N domain-containing protein	-
HV014_RS23350	22686..22958	-	273	WP_000148348.1	helix-turn-helix domain-containing protein	-
HV014_RS23355	23022..23402	-	381	WP_024227067.1	hypothetical protein	-
HV014_RS23360	23670..23954	+	285	WP_001673380.1	hypothetical protein	-
HV014_RS23365	23988..24287	-	300	WP_001673379.1	XRE family transcriptional regulator	Antitoxin
HV014_RS23370	24287..24634	-	348	WP_025667955.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV014_RS23375	24879..25142	-	264	WP_000424604.1	hypothetical protein	-
HV014_RS23380	25166..25453	-	288	WP_024227106.1	hypothetical protein	-
HV014_RS23385	26097..26933	-	837	WP_181199446.1	RepA	-
HV014_RS23390	27307..27480	+	174	WP_181199447.1	tail fiber assembly protein	-
HV014_RS23395	27514..28038	-	525	WP_181199448.1	tail fiber assembly protein	-
HV014_RS23400	28187..28447	-	261	Protein_46	phage tail protein	-
HV014_RS23405	29003..29566	-	564	Protein_47	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..46375	46375

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13530.50 Da
• Isoelectric Point: 9.3634

>T162450 WP_025667955.1 NZ_CP055602:c24634-24287 [Escherichia fergusonii]
MWTVLFSQRFDDWLNEQEDALQEKVLADLKNLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T162450 NZ_CP071794:2339820-2340068 [Parasphingorhabdus cellanae]
ATGGAAAACTTTTCAAACGCTCGTGCCAATCTAAAAGCGCTGATGGACCGTGTTGTCGCCGATCGTGCGCCGGTAAAGAT
CACGCGTCAGCGCGGAGAAGCAGTGGTCATGATAGCCGAAAGCGAATGGGCTGGTATGGAGGAGACGATACATTTGTTAT
CCTCTCCCGCCAACGCCAAAGAACTGTTAGAATCAATCGCACAACTTGATGCCGGCGAGGGCCAGGAACGTGAGCTAATT
GACCCATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11069.90 Da
• Isoelectric Point: 5.8899

>AT162450 WP_001673379.1 NZ_CP055602:c24287-23988 [Escherichia fergusonii]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEAMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT162450 NZ_CP071794:2340065-2340331 [Parasphingorhabdus cellanae]
ATGAAGCTCGCCTTTTCCGAAAAGGCTTGGAATCACTATCTTTACTGGCAAGACAAAGACCGAAAGACGCTCAAGCGTAT
CAACGCTTTGCTGGAAGAATGTCGGCGGCACCCATTTGAGGGCACAGGCAAACCCGAACCCTTGCGCGGCCAGCTAAAAG
GCTGGTGGTCGCGGCGGATTACGCAGGAACACCGACTGGTTTATCGGGTACGCGGCGAAGGTGGCGCCCAAGTGTTGGAA
GTGGCGCAGTGCCGGTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A3P1YQM6