Detailed information of TA system TA162446

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 68248..68512
• Replicon: plasmid pRHB41-C23_3
• Accession: NZ_CP055601
• Organism: Escherichia fergusonii strain RHB41-C23

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HV014_RS22935
• Protein ID: WP_001331364.1
• Coordinates: 68360..68512 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	68248..68305 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV014_RS22920	64290..65360	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HV014_RS22925	65379..66587	-	1209	WP_001383960.1	IncI1-type conjugal transfer protein TrbA	-
HV014_RS22930	66805..67791	-	987	WP_001257838.1	hypothetical protein	-
-	67952..68007	-	56	NuclAT_1	-	-
-	67952..68007	-	56	NuclAT_1	-	-
-	67952..68007	-	56	NuclAT_1	-	-
-	67952..68007	-	56	NuclAT_1	-	-
-	68248..68305	-	58	NuclAT_0	-	Antitoxin
-	68248..68305	-	58	NuclAT_0	-	Antitoxin
-	68248..68305	-	58	NuclAT_0	-	Antitoxin
-	68248..68305	-	58	NuclAT_0	-	Antitoxin
HV014_RS22935	68360..68512	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HV014_RS22940	68584..68835	-	252	WP_001291965.1	hypothetical protein	-
HV014_RS22945	69494..69670	-	177	WP_001054897.1	hypothetical protein	-
HV014_RS22950	69879..70088	-	210	WP_052939150.1	hemolysin expression modulator Hha	-
HV014_RS22955	70186..70800	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
HV014_RS22960	70876..73044	-	2169	WP_063267537.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA1 / blaTEM-1B / sul2	-	1..112380	112380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T162446 WP_001331364.1 NZ_CP055601:68360-68512 [Escherichia fergusonii]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T162446 NZ_CP071793:c5300326-5299901 [Sulfidibacter corallicola]
TTGACGCGGCTAATCATGCTGGACACCAACACCTGCAGCTTCATCATGCGGAAGCGACCGCCCACCATTCTCGAAACCAT
GACTCGATACGTCCTCGAAGGAAACCTCCTCGTCATTTCGGCAATCACGTACTTGGAACTGAGATATGGCGCCATCGGCA
AAAAGGCGAGTCCGAAACACAACTTGCTTGTAACCGAGTTCGTCGATCGAATCGACGAGGTCGTCGGCTTCGATAGAGAA
GCCGTGGAATCCACGGCCCGCTTGAAAAAACACCTTTCGGATCGAGGAACGCCGATTTGCCCCAACGACACCCTCATCGC
CGGTCACGCCCTCGCGATCGATGCGGTGCTGGTGACCGACAACCTGGCGGAGTTCGGCCGAGTTCCCGATTTGGAACTGG
AGAACTGGAAGACCGGCTCCCAATGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT162446 NZ_CP055601:c68305-68248 [Escherichia fergusonii]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin