Detailed information of TA system TA162423

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 965042..965267
• Replicon: chromosome
• Accession: NZ_CP055599
• Organism: Escherichia fergusonii strain RHB41-C23

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: HV014_RS04710
• Protein ID: WP_000813254.1
• Coordinates: 965042..965197 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	965209..965267 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV014_RS04675	960275..960661	-	387	WP_023155117.1	phage holin family protein	-
HV014_RS04680	960828..961688	-	861	WP_048949149.1	hypothetical protein	-
HV014_RS04685	961822..962511	-	690	WP_181199096.1	antiterminator	-
HV014_RS04690	962508..962867	-	360	WP_089585352.1	RusA family crossover junction endodeoxyribonuclease	-
HV014_RS04695	962880..963929	-	1050	WP_048949148.1	DUF968 domain-containing protein	-
HV014_RS04700	963931..964209	-	279	WP_024175747.1	hypothetical protein	-
HV014_RS04705	964506..964898	-	393	WP_000737636.1	hypothetical protein	-
HV014_RS04710	965042..965197	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
-	965209..965267	+	59	-	-	Antitoxin
HV014_RS04715	965435..966100	-	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
HV014_RS04720	966275..966700	-	426	WP_097330215.1	DUF977 family protein	-
HV014_RS04725	966741..967811	-	1071	WP_181199097.1	phage replisome organizer	-
HV014_RS04730	967883..968308	-	426	WP_000693850.1	Rha family transcriptional regulator	-
HV014_RS04735	968305..968520	-	216	WP_060615121.1	transcriptional regulator	-
HV014_RS04740	968570..969286	+	717	WP_001394126.1	helix-turn-helix domain-containing protein	-
HV014_RS04745	969559..969714	+	156	WP_000379589.1	DUF1391 family protein	-
HV014_RS04750	969874..970092	+	219	WP_001171942.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	tssA	935838..984289	48451

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T162423 WP_000813254.1 NZ_CP055599:c965197-965042 [Escherichia fergusonii]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T162423 NZ_CP071772:c74466-74176 [Acinetobacter towneri]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCGCTCAAAACTGAAGAAGACATTGTGATGTATCTCAA
TATGGTTCTTGAAGAAAATGATCCAGCTGAATTAGCCCATGCACTTGGTGTTATTGCTAAAGCTCGTGGCATGACTCAAA
TCGCTAAAGAAGCAGGGATTGGGCGTGAAGCACTCTACAAAGCCCTACGTCAGGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTCACAGTACAACACGCTTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT162423 NZ_CP055599:965209-965267 [Escherichia fergusonii]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin