Detailed information of TA system TA162365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4957604..4958364
• Replicon: chromosome
• Accession: NZ_CP055418
• Organism: Citrobacter sp. RHBSTW-00944

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7L6U900
• Locus tag: HV334_RS24090
• Protein ID: WP_019077936.1
• Coordinates: 4957604..4957915 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV334_RS24095
• Protein ID: WP_141876133.1
• Coordinates: 4957912..4958364 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV334_RS24060	4953271..4954173	+	903	WP_181220990.1	formate dehydrogenase O subunit beta	-
HV334_RS24065	4954170..4954805	+	636	WP_003028686.1	formate dehydrogenase cytochrome b556 subunit	-
HV334_RS24070	4954802..4955731	+	930	WP_003028685.1	formate dehydrogenase accessory protein FdhE	-
HV334_RS24075	4955768..4956142	-	375	WP_016155182.1	type II toxin-antitoxin system VapC family toxin	-
HV334_RS24080	4956142..4956384	-	243	WP_016155181.1	ribbon-helix-helix domain-containing protein	-
HV334_RS24085	4956590..4957519	+	930	WP_181220991.1	alpha/beta hydrolase	-
HV334_RS24090	4957604..4957915	+	312	WP_019077936.1	type II toxin-antitoxin system HigB family toxin	Toxin
HV334_RS24095	4957912..4958364	+	453	WP_141876133.1	helix-turn-helix domain-containing protein	Antitoxin
HV334_RS24100	4958382..4959323	-	942	WP_003825286.1	fatty acid biosynthesis protein FabY	-
HV334_RS24105	4959368..4959805	-	438	WP_003028671.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HV334_RS24110	4959802..4960674	-	873	WP_019077938.1	virulence factor BrkB family protein	-
HV334_RS24115	4960668..4961267	-	600	WP_137351793.1	glucose-1-phosphatase	-
HV334_RS24120	4961535..4962563	-	1029	WP_047357782.1	LacI family DNA-binding transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4947022..4958364	11342

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (95-148)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12502.67 Da
• Isoelectric Point: 9.9988

>T162365 WP_019077936.1 NZ_CP055418:4957604-4957915 [Citrobacter sp. RHBSTW-00944]
MHVISRKPFNEAILRFPNHMAALVDLLNILEKKMFHTPEEMKQYIPSLDNFKYRNKWWVINVSGNCLRLIAYIDFKLQKV
FVKHIVHHAEYDRLTTYYRGHKE

Nucleotide

• Length: 312 bp

>T162365 NZ_CP071758:c886900-886697 [Burkholderia pseudomallei]
TTGCAGCGCAACTCGACTGGTCAGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGG
GTTCGGTGTGATCGGCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTA
AGGCGAACAAGCGGGAGGCCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16998.39 Da
• Isoelectric Point: 5.9259

>AT162365 WP_141876133.1 NZ_CP055418:4957912-4958364 [Citrobacter sp. RHBSTW-00944]
MRTHESHQIDTASVKLMIDTFTDAVKKIPLLGKEQNETEYRKALALVEFLVDRDDLENPLFELLSARIRDYEKHAPEFSA
LNQQLEHTPHGVALLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVSHIKMLAERFNLPADAFIE

Nucleotide

• Length: 453 bp

>AT162365 NZ_CP071758:c886713-886429 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACCGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGCATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAACCTGCGTAG

Structures

Toxin

• AlphaFold DB: A0A7L6U900