Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 1660880..1662312 Replicon chromosome
Accession NZ_CP055306
Organism Mannheimia pernigra strain BNO311

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag HV559_RS08070 Protein ID WP_176810077.1
Coordinates 1660880..1661221 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HV559_RS08075 Protein ID WP_176810078.1
Coordinates 1661233..1662312 (+) Length 360 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV559_RS08030 1655998..1656450 - 453 WP_176810069.1 DUF1367 family protein -
HV559_RS08035 1656471..1657043 - 573 WP_176810070.1 adenine methyltransferase -
HV559_RS08040 1657040..1658401 - 1362 WP_176810071.1 replicative DNA helicase -
HV559_RS08045 1658398..1659261 - 864 WP_176810072.1 replication protein -
HV559_RS08050 1659236..1659406 - 171 WP_176810073.1 hypothetical protein -
HV559_RS08055 1659487..1659732 - 246 WP_176810074.1 hypothetical protein -
HV559_RS08060 1659753..1659953 - 201 WP_176810075.1 hypothetical protein -
HV559_RS08065 1660087..1660764 + 678 WP_176810076.1 helix-turn-helix domain-containing protein -
HV559_RS08070 1660880..1661221 + 342 WP_176810077.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HV559_RS08075 1661233..1662312 + 1080 WP_176810078.1 HigA family addiction module antidote protein Antitoxin
HV559_RS08085 1663650..1664462 - 813 WP_176810079.1 hypothetical protein -
HV559_RS08090 1664680..1664943 + 264 WP_176810080.1 hypothetical protein -
HV559_RS08095 1665224..1665898 + 675 WP_176810081.1 Bro-N domain-containing protein -
HV559_RS08100 1666000..1666143 + 144 WP_176807315.1 hypothetical protein -
HV559_RS08105 1666140..1666505 + 366 WP_176810082.1 helix-turn-helix domain-containing protein -
HV559_RS08110 1666498..1666635 + 138 WP_176810083.1 hypothetical protein -
HV559_RS08115 1666635..1666889 + 255 WP_176810398.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1626577..1675916 49339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-84)

Antitoxin

Cro/C1-type helix-turn-helix domain

(15-72)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12969.25 Da        Isoelectric Point: 9.8274

>T162330 WP_176810077.1 NZ_CP055306:1660880-1661221 [Mannheimia pernigra]
MNIIFKSKKLEKILNSDTEIQKHYGKICGKKIKILLYSLKAAPTLQEFYPPLSKPYRCHELLGKRKGEISMDVEHPYRLL
FEVNNTPLPTLDAGGLDWSKVTSIRILSITDTH

Download         Length: 342 bp

>T162330 NZ_CP071733:c2548120-2547866 [Bifidobacterium longum subsp. infantis]
ATGCTGCTCTGCTGGACCGAAGACGCCTGGGCCGACTACCTGTACTGGCAATCCCAGGACCGCAAGACCCTCAAACGCGT
CAACACGCTCATCCGGGACATGCAGCGAACCCCGTTCGAAGGCATCGGCAAGCCCGAACCCCTCAAATGGGGCCTGTCCG
GCGCATGGTCGCGGCGCATCGATTCGGCCAACCGCATCATCTACACCGTCGCCGACGACAGGCTCTGCACCCTCTCCGCG
AAGGACCACTACTGA

Antitoxin


Download         Length: 360 a.a.        Molecular weight: 41071.99 Da        Isoelectric Point: 8.6835

>AT162330 WP_176810078.1 NZ_CP055306:1661233-1662312 [Mannheimia pernigra]
MSENQFYPDYAILPGEILEFELDSLEMSQKDLSDKTGISTKHISNIINGKAPITPETAILLERVLGKSSRYWINLEALYQ
EATARLADKEKLARYAEWAKQFPNSMLQKLGFIEKHKDISNKVEALLRFFGLGSPDDYQTVWGKLQVQYRQDNRTKNCPH
SSAAWLRAGEIEANKISCNPYSEKDFKKALSKIRALTKITDPQTFIPKLQEICANVGVAVVFIPCFPNTGISGATRWLSS
DKAVVQLSLRYKTNDHLWFTFFHEAAHILLHGKKAVYLEFNSKNSNLEEENEANDFAQKQLIPREVWRLIIKHPLTESSI
KSIAKEIGIAEGIIVGQLQHHQYLSYQSLNHLKVRYTWT

Download         Length: 1080 bp

>AT162330 NZ_CP071733:c2548389-2548120 [Bifidobacterium longum subsp. infantis]
ATGATGACACAGGCCGTGGCCTACAGCAATTTCAGGGCGAATCTCAAGACCTACATGCGCAGGGTCAACGAGGACGCCGA
TACCCTTCTCGTGACCAACGCGGACCCGGAGGACAACGTGGTCGTGATGAGCGCCGACGACTACGATTCGCTCATGGAGA
CGCTGCGCGTCTACCAGAACCCGTATCTGAGCGATAAGGTGATGCGCGGCATGGCGCAGGTCCGGCAGGGCCAGACGATG
GCCCACGACCTTGTCGAAGCGGGCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References