Detailed information of TA system TA162329

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1654290..1654928
• Replicon: chromosome
• Accession: NZ_CP055306
• Organism: Mannheimia pernigra strain BNO311

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HV559_RS08015
• Protein ID: WP_176810066.1
• Coordinates: 1654290..1654595 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HV559_RS08020
• Protein ID: WP_176810067.1
• Coordinates: 1654608..1654928 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV559_RS07955	1649699..1650223	-	525	WP_176810058.1	terminase small subunit	-
HV559_RS07960	1650589..1650846	-	258	WP_176810059.1	hypothetical protein	-
HV559_RS07965	1650847..1651071	-	225	WP_176810060.1	hypothetical protein	-
HV559_RS07970	1651016..1651366	-	351	WP_176810061.1	DUF2570 domain-containing protein	-
HV559_RS07975	1651339..1651878	-	540	WP_176810062.1	lysozyme	-
HV559_RS07980	1651875..1652120	-	246	WP_176808239.1	hypothetical protein	-
HV559_RS07985	1652233..1652811	+	579	WP_176810063.1	hypothetical protein	-
HV559_RS08000	1653098..1653571	-	474	WP_176810064.1	hypothetical protein	-
HV559_RS08005	1653561..1653887	-	327	WP_201723603.1	RusA family crossover junction endodeoxyribonuclease	-
HV559_RS08010	1653926..1654210	-	285	WP_176810065.1	DUF1364 domain-containing protein	-
HV559_RS08015	1654290..1654595	+	306	WP_176810066.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV559_RS08020	1654608..1654928	+	321	WP_176810067.1	HigA family addiction module antidote protein	Antitoxin
HV559_RS08025	1655263..1655940	+	678	WP_176810068.1	phage repressor protein	-
HV559_RS08030	1655998..1656450	-	453	WP_176810069.1	DUF1367 family protein	-
HV559_RS08035	1656471..1657043	-	573	WP_176810070.1	adenine methyltransferase	-
HV559_RS08040	1657040..1658401	-	1362	WP_176810071.1	replicative DNA helicase	-
HV559_RS08045	1658398..1659261	-	864	WP_176810072.1	replication protein	-
HV559_RS08050	1659236..1659406	-	171	WP_176810073.1	hypothetical protein	-
HV559_RS08055	1659487..1659732	-	246	WP_176810074.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1626577..1675916	49339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-67)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12024.57 Da
• Isoelectric Point: 5.7064

>T162329 WP_176810066.1 NZ_CP055306:1654290-1654595 [Mannheimia pernigra]
MFNLTEAHFRDEALYRFFQYGETSRTIPANLTNVLARKLDMINAAENLNDLRSPPANRLELLEPKQNNIYSIRVNKQYRL
IFKFENNELSDLYLDPHNYDL

Nucleotide

• Length: 306 bp

>T162329 NZ_CP071733:2199940-2200167 [Bifidobacterium longum subsp. infantis]
ATGAAGAAGCTGGATCGTAATGTTGCCAAGAGAATCATCGCAAAGCTTAGGGAAATTTCACAGTTGGAAGATCCGAGAAG
CACCGGCAAGGCTCTTGCCGGTAATCTGGCAGGGCTGTGGCGTTACCGTGTCGGGGACTATCGTATCGTGTGCGATATAG
AGGATGAGGTTCTGCTGATTCTTGTGATTGATGTCGCGCACAGGAGCAAGGTGTACAAGCGTTGCTGA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12078.80 Da
• Isoelectric Point: 6.7167

>AT162329 WP_176810067.1 NZ_CP055306:1654608-1654928 [Mannheimia pernigra]
MMQRKPTPVGEILQEEFLEPLNLKIGDLADILGVHRNTASNIVNNSCRVSPEMAFKLAKAFETSPEFWLNLQTASDLWEL
RHNNRFQQSLNSVKAVNHWQGMPAFA

Nucleotide

• Length: 321 bp

>AT162329 NZ_CP071733:2199678-2199914 [Bifidobacterium longum subsp. infantis]
ATGGCCACGGTTACTGCAGCATTAAGGATGCCTGTCGAATTGGCGGCTCGTTACGATTGTCTGGCGAAAGCGACTGGTCG
TACGAAGACGTTTTACATGAACGAAGCCCTTACCGAATCGATCGATAGGTTTGAGTATGAATACGGCATTATGAAAAAGG
TCGAGGATTGGCGTGCCGGCAGACTCGAAACCGTGACGCTCGACGAGCTGGAGGAAAGCCTTGTCTTGGAGGATTGA